Laboratories and faculty

Molecular Microbiology and Genetics

Outline of Research and Education

Global warming resulting from elevated CO2 and global energy supply problems have been in the limelight in recent years. As these problems originate from rapid economic expansion and regional instability in parts of the world, broad knowledge of global economic systems as well as R&D is necessary to solve these problems. Fundamental research employing microbial functions to tackle adverse effects of global climate change and mitigate energy supply problems is carried out in our laboratory.

Major Research Topics

Biorefinery

A biorefinery is a concept which describes production of chemicals and fuels from renewable biomass via biological processes. Biorefinery R&D is considered of national strategic importance to the U.S.A. (Fig. 1). A biorefinery can be divided into two processes: a saccharification process to hydrolyze biomass to sugars, and a bioconversion process to produce chemicals and fuels from the sugars. We have pioneered, as a bioconversion technology to produce chemicals and fuels, a highly-efficient “growth-arrested bioprocess” based on a novel concept (Fig.2). It is based on Corynebacteria that are widely used in industrial amino acid production. Key to the high efficiency is the productivity of artificially growth-arrested microbial cells, cells with which we are evaluating production of organic acids and biofuels. To efficiently produce these products, the cells are tailored for the production of a particular product using post genome technologies like transcriptomics, proteomics and metabolome analyses (Fig.3).

Bioenergy and green chemicals production

Having established the fundamental technology to produce bioethanol from non-food biomass, we are now partnering with the automobile and petrochemical industries to explore commercial application. We have also developed the platform technology to produce biobutanol, the expected next-generation biofuel, as well as a variety of green chemicals such as organic acids, alcohols and aromatic compounds from which diverse polymer raw materials used in various industries are produced.

Fig. 1
Fig. 1 Biorefinery concept
Fig. 2
Fig. 2 Novel features of the RITE Bioprocess
Fig. 3
Fig. 3 Breeding of recombinant strains using system biology

References

  1. Nakazawa S. et al., ACS Synth Biol, 10, 2308-2317, 2021
  2. Toyoda K. et al., Microorganisms, 9, 550, 2021
  3. Jojima T. et al., Biotechnol Biofuels, 14, 45, 2021
  4. Kogure T. et al., Metab Eng, 65, 232-242, 2021
  5. Uchikura H. et al., Appl Microbiol Biotechnol, 104, 6719-6729, 2020
  6. Hasegawa S. et al., Metab Eng, 59, 24-35, 2020
  7. Shimizu T. et al., Appl Microbiol Biotechnol, 103, 9739-9749, 2019
  8. Han S.O. et al., Biotechnol J, 14, e1900160, 2019
  9. Tsuge Y. et al., Appl Microbiol Biotechnol, 103, 3381-3391, 2019
  10. Oide S. et al., Enzyme Microb Technol, 125, 13-20, 2019
  11. Shimizu T. et al., Appl Environ Microbiol, 85, e01873-18, 2019
  12. Tsuge Y. et al., J Biosci Bioeng, 127, 288-293, 2019
  13. Kogure T. et al., Appl Microbiol Biotechnol, 102, 8685-8705, 2018
  14. Maeda T. et al., Mol Microbiol, 108, 578-594, 2018
  15. Hasegawa S et al., J Microbiol Methods, 146, 13-15, 2018
  16. Kitade Y. et al., Appl Environ Microbiol, 84, e02587-17, 2018
  17. Toyoda K. et al., Mol Microbiol, 107, 312-329, 2018
  18. Oide S. et al., FEBS J, 284, 4298-4313, 2017
  19. Kuge T. et al., Appl Microbiol Biotechnol, 101, 5019-5032, 2017
  20. Maeda T. et al., J Bacteriol, 199, e00798-16, 2017
  21. Hasegawa S. et al., Appl Environ Microbiol, 83, e02638-16, 2017