Understanding Tolerance Phenotypes in Escherichia coli: Developing the Resistome

演題

Understanding Tolerance Phenotypes in Escherichia coli: Developing the Resistome

講演者

James Winkler
(University of Colorado-Boulder, Department of Chemical and Biological Engineering, Boulder CO USA)

使用言語

English

日時

2015年11月26日(木曜日) 15：00～16：00

場所

Bio-Large seminar room

内容

Metabolic and evolutionary engineers have developed increasingly sophisticated approaches for improving biocatalyst resistance to environmental stresses. However, despite the growing wealth of genetic and phenotypic data characterizing these traits of interest, these data remain scattered amongst the literature, and cannot be used for automated analysis to discover novel (anti)correlations between underlying genetic mechanisms. Designing E. coli strains with multiple desirable tolerance phenotypes, or equivalently designing treatments to prevent dual resistance to antibiotic treatments, remains challenging as a result. We address this deficiency by developing the Resistome, a database containing hundreds of E. coli tolerance-conferring genotypes in a standardized, machine-readable format. In this talk, we examine the genetic bases for these tolerance traits, while introducing computational tools that can automatically infer synergy or antagonism between traits of interest. We anticipate that the Resistome will become a powerful tool for metabolic engineers to design and analyze multi-tolerant strain designs, and enable the development of novel dual treatment strategies to forestall evolution of drug resistance in a medical context.

問合せ先

システム微生物学
森　浩禎 (hmori@gtc.naist.jp)