NAIST 奈良先端科学技術大学院大学 バイオサイエンス領域


Halotag protein array mapping of transcription factor interactome networks

演題 Halotag protein array mapping of transcription factor interactome networks
講演者 矢崎 潤史 博士(理化学研究所横浜研究所 上級研究員)
使用言語 日本語
日時 2013年7月30日(火曜日) 15:00~16:00
場所 L12会議室
Protein microarrays are able to investigate diverse biochemical properties for thousands of proteins in a single experiment, an unparalleled capacity. Using a high-affinity protein microarray approach we developed for this work, HaloTag nucleic acid programmable protein assay (HT-NAPPA), we created high-density protein arrays comprising 12,000 Arabidopsis ORFs. We used these arrays to query protein-protein interactions for a set of 38 transcription factors that function in diverse plant hormone regulatory pathways. The resulting transcription factor interactome network, TF-NAPPA, contains thousands of novel interactions. Evaluation of the quality of TF-NAPPA by in vitro pull-down assays found that 63% of interactions were reproducible.By bimolecular fluorescence complementation (BiFC) assays, we observed co-localization and biophysical interactions for 25% of the protein-pairs examined.
The application of HT-NAPPA technology to hormone signal pathways allowed the identification of many novel transcription factor protein interactions, and led to the development of a proteome-wide plant hormone TF interactome network.
問合せ先 植物代謝制御
出村 拓 (