NAIST 奈良先端科学技術大学院大学 バイオサイエンス領域


Mitotic cell rounding: ramping up the pressure

演題 Mitotic cell rounding: ramping up the pressure
講演者 Dr. Martin Stewart(Department of Biosystems Science and Engineering, ETH Zürich)
使用言語 English
日時 2013年5月8日(水曜日) 17:00~18:00
場所 Large seminar room
Upon entry into mitosis, most animal cells undergo a major morphological transition into spherical shape. This shape change, known as mitotic cell rounding (MCR) has recently been demonstrated to serve important functions in cell division and tissue organization. My talk will summarize the state of knowledge in this small (~50 papers) field and present key results from our laboratory. Over the last 5 years, our laboratory has taken a biophysical approach to investigating the mechanics of MCR by using atomic force microscopy (AFM) in combination with advanced live cell imaging. AFM-¬‐based mechanical measurements with tipless cantilevers have demonstrated that cells possess an intracellular pressure that increases more than three-¬‐fold upon entry into mitosis and correlates with MCR. By verifying models of cell shape based on the law of Laplace, we show that this pressure is related to actomyosin-¬‐based surface tension, and demonstrate how this pressure is linked with dynamic distribution of F-¬‐actin and myosin II trans-¬‐mitosis. Furthermore, to investigate other novel genes that might be involved in MCR, we have also undertaken mechanical phenotyping of mitotic cells with RNAi screening and have identified a number of key proteins. This talk will summarize how changes in mechanical parameters such as intracellular pressure, surface tension and adhesion, as dictated by the activities of cytoskeletal, membrane and regulatory proteins, converge to drive MCR.

Stewart, M. P. et al., A. Hydrostatic pressure and the actomyosin cortex drive mitotic cell rounding. Nature, 469, 226-230, (2011).
 Stewart, M. P. et al., Force probing cell shape changes to molecular resolution. Trends in Biochemical Science, 36, 444-450, (2011).
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