Collagen polarization promotes epithelial elongation by stimulating locoregional cell proliferation
- 演題
- Collagen polarization promotes epithelial elongation by stimulating locoregional cell proliferation
- 講演者
- 勝野 弘子 博士(Dr. Hiroko Katsuno-Kambe)
(Institute for Molecular Bioscience, The University of Queensland in Australia)
- 使用言語
- English
- 日時
- 2021年12月9日(木曜日) 13:30~14:30
- 場所
- Large seminar room
- 内容
Epithelial networks are commonly generated by processes where multicellular aggregates elongate and branch. Here we focus on understanding cellular mechanisms for elongation, using an organotypic culture system as a model of mammary epithelial anlage. Isotropic cell aggregates broke symmetry and slowly elongated when transplanted into collagen 1 gels. The elongating regions of aggregates displayed enhanced cell proliferation that was necessary for elongation to occur. Strikingly, this loco-regional increase in cell proliferation occurred where collagen 1 fibrils reorganized into bundles which were polarized with the elongating aggregates. Applying external stretch as a cell-independent way to reorganize the ECM, we found that collagen polarization stimulated regional cell proliferation to precipitate symmetry-breaking and elongation. This required b1-integrin and ERK signaling. We propose that collagen polarization supports epithelial anlagen elongation by stimulating loco-regional cell proliferation. This could provide a long-lasting structural memory of the initial axis that is generated when anlage break symmetry.
【Reference】
Katsuno-Kambe, H., Teo, J. L., Ju, R. J., Hudson J., Stehbens, S. J., Yap, S. A. (2021) Collagen polarization promotes epithelial elongation by stimulating locoregional cell proliferation. eLife, 10:e67915.- 問合せ先
- Systems Neurobiology and Medicine
Naoyuki Inagaki (ninagaki@bs.naist.jp)