セミナー情報

セミナー情報

Differential Metabolomics for Assessment of N-Acetyl-L-Cysteine Pretreatment in Strenuous Exercise: A Quantitative Model of Oxidative Stress

演題 Differential Metabolomics for Assessment of N-Acetyl-L-Cysteine Pretreatment in Strenuous Exercise: A Quantitative Model of Oxidative Stress
講演者 Dr. Philip Brits-McKibbin (Department of Chemistry and Chemical Biology McMaster University)
使用言語 English
日時 平成22年6月10日(木曜日) 10:00~
場所 バイオサイエンス研究科 大セミナー室
内容

Despite several decades of active research, the success of large-scale clinical trials involving antioxidants remains equivocal given the complex biological interactions of reactive oxygen/nitrogen species in human health.

Herein, we outline a differential metabolomics strategy by capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) to assess the efficacy of nutritional intervention to attenuate oxidative stress induced by strenuous exercise. A healthy volunteer was recruited to perform a submaximal prolonged ergometer cycling trial until volitional exhaustion with frequent blood collection over a 6 h time interval, which included pre-, during, and post-exercise periods while at rest. A follow-up study was subsequently performed by the same subject after high-dose oral intake of N-acetyl-l-cysteine (NAC) prior to performing the same exercise protocol under standardized conditions. Time-dependent changes in global metabolism of filtered red blood cell lysates by CE-ESI-MS were measured to reveal a significant attenuation of cellular oxidation associated with high-dose oral NAC intake relative to a control. Untargeted metabolite profiling allowed for the identification and quantification of several putative early- and late-stage biomarkers that reflected oxidative stress inhibition due to nutritional intervention.

Our work demonstrates the proof-of-principle that NAC pretreatment is effective at dampening acute episodes of oxidative stress by reversible perturbations in global metabolism that can provide deeper insight into the mechanisms of thiol-specific protein inhibition relevant to its successful translation as a prophylaxis in clinical medicine.

問合せ先 生体情報学
森 浩禎 (hmori@gtc.naist.jp)

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