セミナー情報

セミナー情報

Exploitation of the unique reaction in microbial meroterpenoid biosynthesis

演題 Exploitation of the unique reaction in microbial meroterpenoid biosynthesis
講演者 Dr. Takayoshi Awakawa (Assistant Professor, Graduate School of Pharmaceutical Sciences, The University of Tokyo)
使用言語 English
日時 平成29年4月24日(月曜日) 15:00~16:00
場所 Seminar Room L12
内容 Many different kinds of secondary metabolites have been isolated from microorganism so far. To study their biosynthetic reactions is important, because the secondary metabolites exhibit various bioactivities against the others and the biosynthetic study leads to the exploitation of useful bioactive compounds including pharmaceutical agents. In this presentation, two main topics of the biosynthetic study on the microbial meroterpenoids, a group of hybrid compounds consisting of terpenoid and other biosynthetic origin, will be discussed. In both cases, the heterologous gene expression system in microorganisms played a key role to elucidate the biosynthetic pathway. First, the biosynthesis of fungal meroterpenoids, consisting of polyketide and terpenoid, will be presented. The gene expression system in Aspergillus oryzae enabled us to explore the catalyses of unique terpene cyclase and oxidase.1-2) With this powerful gene expression system, now we can efficiently explore the new biosynthetic genes found with bioinformatic analysis of genome database. Secondly, the biosynthesis of actinomycete meroterpenoid with indole and terpenoid, will be presented. The screening method based on the multiple gene expression system successfully identified one key biosynthetic genes located outside of the gene cluster.3) In vitro assay with synthetic substrate revealed unprecedented terpene cyclization reaction triggered by methylation. With complexed with the structural studies of enzyme, these studies paved the way to create unnatural bioactive meroteroenoids.4) 1) Matsuda, Y.; Awakawa, T.; Itoh, T.; Wakimoto, T.; Kushiro, T.; Fujii, I.; Ebizuka, Y.; Abe, I. ChemBioChem 2012, 13, 1738. 2) Matsuda, Y.; Awakawa, T.; Wakimoto, T.; Abe, I. J. Am. Chem. Soc. 2013, 135, 10962. 3) Awakawa, T.; Zhang, L.; Wakimoto, T.; Hoshino, S.; Mori, T.; Ito, T.; Ishikawa, J.; Tanner, M. E.; Abe, I. J. Am. Chem. Soc. 2014, 136, 9910. 4) Mori, T.; Zhang, L.; Awakawa, T.; Hoshino, S.; Okada, M.; Morita, H.; Abe, I. Nat. Commun. 2016, 7, 10849.
問合せ先 ストレス微生物科学
高木 博史 (hiro@bs.naist.jp)

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