セミナー情報

セミナー情報

Omics Approach to Elucidation of Plant Metabolism

演題 Omics Approach to Elucidation of Plant Metabolism
講演者 平井 優美 博士 ((独)理化学研究所 植物科学研究センター)
使用言語 日本語
日時 平成22年10月8日(金曜日) 15:30~
場所 バイオサイエンス研究科 L12会議室
内容

Plants produce chemically-diverse secondary metabolites derived from primary metabolites. In general, primary metabolites including amino acids have fundamental functions in every aspects of life, and hence, primary metabolism is regulated so as to be robust against environmental perturbation. On the other hand, a major role of secondary metabolism is to supply the compounds which have specific biological functions (insect repellents, UV protectants, etc.) in response to environmental stimuli. Hence, secondary metabolism is presumably regulated so as to respond dynamically to environmental changes. To understand plant metabolism and its regulation, we are taking the omics-based strategy. We found that coexpression analysis based on a large-scale transcriptome dataset of Arabidopsis is an effective means in finding the candidate genes involved in secondary metabolism. We revealed a set of the genes involved in biosynthesis of glucosinolates, health-promoting secondary metabolites found mainly in Brassicaceae plants including various vegetables. Modulation of the expression of these genes affected both glucosinolate and amino acid contents, suggesting complicated relationship between amino acid metabolism and secondary metabolism. Recently, we have established a high-throughput metabolic profiling methodology called “widely targeted metabolomics”, which enabled us to acquire a large-scale dataset of metabolic profiles of crops. In this presentation, our recent results based on transcriptomics and widely targeted metabolomics will be introduced .

References:
  1. Hirai MY et al. (2005) Elucidation of gene-to-gene and metabolite-to-gene networks in arabidopsis by integration of metabolomics and transcriptomics. J Biol Chem 280: 25590-25595
  2. Hirai MY et al. (2007) Omics-based identification of Arabidopsis Myb transcription factors regulating aliphatic glucosinolate biosynthesis. Proc Natl Acad Sci U S A 104: 6478-6483
  3. Sawada Y et al. (2009) Widely targeted metabolomics based on large-scale MS/MS data for elucidating metabolite accumulation patterns in plants. Plant Cell Physiol 50: 37-47
  4. Hirai MY et al. (2010) Toward genome-wide metabolotyping and elucidation of metabolic system: metabolic profiling of large-scale bioresources. J Plant Res 123: 291-298
問合せ先 植物代謝調節学
出村 拓 (demura@bs.naist.jp)

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