Outline of Research and Education
Plants continuously produce organs throughout their life. This feature renders them distinct from animals, in which organ formation ceases soon after embryogenesis. We are studying DNA polyploidization and stem cells that support sustained plant growth. We focus on the molecular mechanisms of DNA polyploidization that increase cell volume and organ size, and how plants preserve stem cells. We aim to understand the regulatory system underlying continuous plant growth, and to develop technologies to increase plant biomass and food production.
Major Research Topics
Mechanisms of induction of DNA polyploidization
In many plant species, cells start endoreplication after the cessation of cell division. This is an alternative type of the cell cycle, lacking mitosis and cytokinesis. As a result, DNA content in individual cells is elevated. The resultant DNA polyploidization causes enlargement of individual cells and organs (Fig. 1); thus, it greatly contributes to plant biomass production. However, the induction mechanisms of endoreplication have remained largely unknown. We recently found that regulation of chromatin structure plays essential roles in triggering endoreplication. Therefore, we are studying the epigenetic control of endoreplication, and developing technologies to induce DNA polyploidization, which may increase crop yield and woody biomass production (Fig. 2).
Maintenance of plant stem cells
The sequoia, the largest tree on the earth, has a life span of more than 3,000 years. It continues to grow throughout its lifespan, indicating that pluripotent stem cells function for a long time period under changing environmental conditions. How plants generate, proliferate, and maintain stem cells in tissues, however, remains elusive. We are studying the mechanisms of how the stem cell niche is regenerated through reprogramming, and how stem cells are replenished when they are lost owing to environmental stresses (Fig. 3). Our study will shed light on the pluripotency of stem cells and the process of tissue regeneration.
- Ogita N. et al., Plant J., 94, 439-453, 2018
- Chen P. et al., Nature Commun., 8, 635, 2017
- Ueda M. et al., Genes Dev., 31, 617-627, 2017
- Weimer A.K. et al., EMBO J., 35, 2068-2086, 2016
- Kobayashi K. et al., EMBO J., 34, 1992-2007, 2015
- Takatsuka H. et al., Plant J., 82, 1004-1017, 2015
- Yin K. et al., Plant J., 80, 541-552, 2014
- Takatsuka H. and Umeda M., J. Exp. Bot., 65, 2633-2643, 2014
- Takahashi N. et al., Curr. Biol., 23, 1812-1817, 2013
- Yoshiyama K.O. et al., EMBO Rep., 14, 817-822, 2013
- Nobusawa T. et al., PLOS Biol., 11, e1001531, 2013
- Breuer C. et al., EMBO J., 31, 4488-4501, 2012
- Adachi S. et al., Proc. Natl. Acad. Sci. USA, 108, 10004-10009, 2011
- Takatsuka H. et al., Plant J., 59, 475-487, 2009
- Kono A. et al., Plant Cell, 19, 1265-1277, 2007
- Yamaguchi M. et al., Proc. Natl. Acad. Sci. USA, 100, 8019-8023, 2003
- Umeda M. et al., Proc. Natl. Acad. Sci. USA, 97, 13396-13400, 2000