Japanese

Laboratory of Applied Stress MicrobiologyLaboratory of Applied Stress Microbiology

content ▼

Research Projects

6. Physiological function of microbial enzyme, fructosyl-amino acid oxidase, and its application to the clinical diagnosis of diabetes

Fructosyl-amino acid oxidase (FAOD) catalyzes the oxidative deglycation of the glycated amino acid to produce the corresponding amino acid, glucosone, and H2O2. The FAODs have been used for enzymatic assay for glycated proteins, which are good indices of diabetic mellitus. We examine the physiological role of FAODs from various microorganisms including the gene expression and cellular localization.

  1. N. Yoshida, S. Akazawa, T. Karino, H. Ishida, Y. Hata, T. Katsuragi, Y. Tani and H. Takagi: Functional analysis of genes encoding putative oxidoreductases in Aspergillus oryzae, which are similar to fungal fructosyl-amino acid oxidase. J. Biosci. Bioeng., 104, 424-427 (2007).
  2. N. Yoshida, S. Akazawa, A. Kuwahara, T. Katsuragi and Y. Tani: Fructosyl-amino acid oxidases of Aspergillus oryzae are induced by the reaction product, glucosone. FEMS Microbiol. Lett., 248, 141-145 (2005).
  3. N. Yoshida, K. Takatsuka, T. Katsuragi and Y. Tani: Occurrence of fructosyl-amino acid oxidase-reactive compounds in fungal cells. Biosci. Biotechnol. Biochem., 69, 258-260 (2005).
  4. S. Akazawa, T. Karino, N. Yoshida, T. Katsuragi and Y. Tani: Functional analysis of fructosyl-amino acid oxidases of Aspergillus oryzae. Appl. Environ. Microbiol., 70, 5882-5890 (2004).
  5. N. Yoshida, S. Akazawa, T. Katsuragi and Y. Tani: Characterization of two fructosyl-amino acid oxidase homologs of Schizosaccharomyces pombe. J. Biosci. Bioeng., 97, 278-280 (2004).
  6. Y. Sakai, H. Yoshida, H. Yurimoto, N. Yoshida, H. Fukuya, K. Takabe and N. Kato: Production of fungal fructosyl amino acid oxidase useful for diabetic diagnosis in the peroxisome of Candida boidinii. FEBS Lett., 459, 233-237 (1999).
  7. N. Yoshida, Y. Sakai, A. Isogai, H. Fukuya, M. Yagi, Y. Tani and N. Kato: Primary Structures of fungal fructosyl amino acid oxidases and its application to the measurement of glycated proteins. Eur. J. Biochem., 242, 499-505 (1996).
  8. Y. Sakai, N. Yoshida, Y. Tani and N. Kato: Production of fructosyl lysine oxidase from Fusarium oxysporum S-1F4 on autoclave-browned medium. Biosci. Biotechnol. Biochem., 60, 150-151 (1996).
  9. N. Yoshida, Y. Sakai, M. Serata, Y. Tani and N. Kato: Distribution and properties of fructosyl amino acid oxidase in fungi. Appl. Environ. Microbiol., 61, 4487-4489 (1995).
  10. Y. Sakai, N. Yoshida, A. Isogai, Y. Tani and N. Kato: Purification and properties of fructosyl lysine oxidase from Fusarium oxysporum S-1F4. Biosci. Biotechnol. Biochem., 59, 487-491 (1995).
PageTop