Laboratory of Applied Stress MicrobiologyLaboratory of Applied Stress Microbiology

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Research Projects

1. Novel stress-tolerant mechanisms of yeast cells and their applications for breeding of industrial yeast

We are interested in cellular response and adaptation to environmental stresses using the budding yeast Saccharomyces cerevisiae, which is an important microorganism in basic science as a model for higher eukaryotes. Yeast is also a useful microbe in the fermentation industry for the production of breads, alcoholic beverages and bioethanol. During fermentation, yeast cells are exposed to various stresses, including ethanol, high temperature, desiccation and osmotic pressure. Such stresses induce protein denaturation, reactive oxygen species (ROS) generation, and lead to growth inhibition or cell death. In terms of industrial application, stress tolerance is the key for yeast cells. We analyze novel stress-tolerant mechanisms of yeast as listed below.

oxidative stress, reactive oxygen specise, proline, γ-glutamate kinase, proline oxidase, transporter, mitochondria, N-acetyltransferase Mpr1, arginine, nitric oxide (NO), NO synthase Tah18, S-nitrosylation, redox regualtion, ubiquitin system, ubiquitin ligase Rsp5, regulation of protein activity (ubiquitination, phosphorylation), the transcription factor Pog1, lithium ion stress, TOR signaling pathway