Laboratory of Applied Stress Microbiology

Research Projects

5. Novel CO₂-fixation system in extremely oligotroph Rhodococcus sp.

We have previously isolated an extremely oligotrophic bacterium, Rhodococcus erythropolis N9T-4, from crude oil stored in a Japanese oil stockpile. This bacterium could grow on a minimal salt medium solidified by using agar or silica gel without any additional carbon or energy source, suggesting that this bacterium has a novel and effective CO₂-fixation system or specific metabolism of CO₂. We now examine the genomic, proteomic, and metabolomic analyses to elucidate the CO₂-fixation system, and propose the novel CO₂-fixation system involved in formaldehyde metabolism.

oligotrophic stress, carbon dioxide fixation, methanol metabolism, formaldehyde, oligobody

1. Nobuyuki Yoshida*, Takanori Yano*, Kaori Kedo, Takuya Fujiyoshi, Rina Nagai, Megumi Iwano, Eiji Taguchi, Tomoki Nishida, Hiroshi Takagi: A unique intracellular compartment formed during the oligotrophic growth of Rhodococcus erythropolis N9T-4. *These authors contributed equally to this work. Appl. Microbiol. Biotech., 101, 331-340 (2017).
2. T. Yano*, N. Yoshida*, F. Yu, M. Wakamatsu and H. Takagi: The glyoxylate shunt is essential for CO₂-requiring oligotrophic growth of Rhodococcus erythropolis N9T-4. *These authors contributed equally to this work.Appl. Microbiol. Biotech., 99, 5627-5637 (2015).
3. N. Yoshida, S. Inaba and H. Takagi: Utilization of atmospheric ammonia by an extremely oligotrophic bacterium, Rhodococcus erythropolis N9T-4. J. Biosci. Bioeng, 117, 28-32 (2014).
4. T. Yano, N. Yoshida and H. Takagi: Carbon monoxide utilization of an extremely oligotrophic bacterium, Rhodococcus erythropolis N9T-4. J. Biosci. Bioeng., 114, 53-55 (2012).
5. N. Yoshida, T. Hayasaki and H. Takagi: Gene expression analysis of methylotrophic oxidoreductases involved in the oligotrophic growth of Rhodococcus erythropolis N9T-4. Biosci. Biotech. Biochem., 75, 123-127 (2011).
6. N. Yoshida, N. Ohhata, Y. Yoshino, T. Katsuragi, Y. Tani and H. Takagi: Screening of carbon dioxide-requiring extreme oligotrophs from soil. Biosci. Biotech. Biochem., 71, 2830-2832 (2007).
7. N. Ohhata, N. Yoshida, H. Egami, T. Katsuragi, Y. Tani and H. Takagi: An extremely oligotrophic bacterium, Rhodococcus erythropolis N9T-4 isolated from crude oil. J. Bacteriol., 189, 6824-6831 (2007).
8. N. Yoshida, K. Yagi, D. Sato, N. Watanabe, T. Kuroishi, K. Nishimoto, A. Yanagida, T. Katsuragi, T. Kanagawa, R. Kurane and Y. Tani: Bacterial communities in petroleum oil in stockpiles. J. Biosci. Bioeng., 99, 143-149 (2005).