業績リスト

原著論文

  1. Wangpaiboon K, Laohawuttichai P, Kim SY, Mori T, Nakapong S, Pichyangkura R, Pongsawasdi P, Hakoshima T, Krusong, K. 
    A GH13 α-glucosidase from Weissella cibaria uncommonly acts on short-chain maltooligosaccharides
    Acta Crystallogr D Struct Biol. 2021 Aug;77(Pt 8):1064-1076. doi: 10.1107/S205979832100677X.

  2. Lim H, Chuah JA, Chek MF, Tan HT, Hakoshima T, Sudesh K.
    Identification of regions affecting enzyme activity, substrate binding, dimer stabilization and polyhydroxyalkanoate (PHA) granule morphology in the PHA synthase of Aquitalea sp. USM4. 
    Int J Biol Macromol. 2021 Jul 8;186:414-423. doi: 10.1016/j.ijbiomac.2021.07.041. Epub ahead of print. PMID: 34246679.

  3. Kim SY, Mori T, Chek MF, Furuya S, Matsumoto K, Yajima T, Ogura T, Hakoshima T. 
    Structural insights into vesicle amine transport-1 (VAT-1) as a member of the NADPH-dependent quinone oxidoreductase family. 
    Sci Rep. 2021 Jan 22;11(1):2120. doi: 10.1038/s41598-021-81409-y. PMID: 33483563; PMCID: PMC7822847.

  4. Murase K, Moriwaki Y, Mori T, Liu X, Masaka C, Takada Y, Maesaki R, Mishima M, Fujii S, Hirano Y, Kawabe Z, Nagata K, Terada T, Suzuki G, Watanabe M, Shimizu K, Hakoshima T, Takayama S.
    Mechanism of self/nonself-discrimination in Brassica self-incompatibility.
    Nat Commun. 2020 Oct 1;11(1):4916. doi: 10.1038/s41467-020-18698-w.PMID: 33004803

  5. Tanaka Y, Yoshikaie K, Takeuchi A, Ichikawa M, Mori T, Uchino S, Sugano Y, Hakoshima T, Takagi H, Nonaka G, Tsukazaki T.
    Crystal structure of a YeeE/YedE family protein engaged in thiosulfate uptake.
    Sci Adv. 2020 Aug 26;6(35):eaba7637. doi: 10.1126/sciadv.aba7637. eCollection 2020 Aug.PMID: 32923628

  6. Tan HT, Chek MF, Lakshmanan M, Foong CP, Hakoshima T, Sudesh K.
    Evaluation of BP-M-CPF4 Polyhydroxyalkanoate (PHA) Synthase on the Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) From Plant Oil Using Cupriavidus Necator Transformants.
    Int J Biol Macromol 2020 May 14;159:250-257. doi: 10.1016/j.ijbiomac.2020.05.064. Online ahead of print.

  7. Chek MF, Kim SY, Mori T, Tan HT, Sudesh K, Hakoshima T.
    Asymmetric open-closed dimer mechanism of polyhydroxyalkanoate (PHA) synthase PhaC.
    iScience 2020 May 22;23(5):101084.doi: 10.1016/j.isci.2020.101084. Epub 2020 Apr 21.

  8. Furutani M, Hirano Y, Nishimura T, Nakamura M, Taniguchi M, Suzuki K, Oshida R, Kondo C, Sun S, Kato K, Fukao Y, Hakoshima T,  Morita MT
    Polar recruitment of RLD by LAZY1-like protein during gravity signaling in root branch angle control.
    Nature Commun. 2020;11(1):76. Published 2020 Jan 3. doi:10.1038/s41467-019-13729-7

  9. Chek MF, Hiroe A, Hakoshima T, Sudesh K, Taguchi S.
    PHA synthase (PhaC): interpreting the functions of bioplastic-producing enzyme from a structural perspective.
    Appl Microbiol Biotechnol. 2019 Feb;103(3):1131-1141. doi:10.1007/s00253-018-9538-8. Epub 2018 Dec 3. Review.

  10. Seto Y, Yasui R, Kameoka H, Tamiru M, Cao M, Terauchi R, Sakurada A, Hirano R, Kisugi T, Hanada A, Umehara M, Seo E, Akiyama K, Burke J, Takeda-Kamiya N, Li W, Hirano Y, Hakoshima T, Mashiguchi K, Noel JP, Kyozuka J, Yamaguchi S.
    Strigolactone perception and deactivation by a hydrolase receptor DWARF14.
    Nature Commun. 2019 Jan 14;10(1):191. doi: 10.1038/s41467-018-08124-7.

  11. Kim SY, Hakoshima T.
    GST Pull-Down Assay to Measure Complex Formations.
    Methods Mol Biol. 2019; 1893:273-280. doi: 10.1007/978-1-4939-8910-2_20.

  12. Hirano Y, Amano Y, Yonemura S, Hakoshima T.
    The force-sensing device region of α-catenin is an intrinsically disordered segment in the absence of intramolecular stabilization of the autoinhibitory form.
    Genes Cells. 2018 May;23(5):370-385. doi: 10.1111/gtc.12578.

  13. Kimata S, Mochizuki D, Satoh J, Kitano K, Kanesaki Y, Takeda K, Abe A, Kawasaki S, Niimura Y.
    Intracellular free flavin and its associated enzymes participate in oxygen and iron metabolism in Amphibacillus xylanus lacking a respiratory chain.
    FEBS Open Bio. 2018 May 9;8(6):947-961. doi: 10.1002/2211-5463.12425.

  14. Abe K, Katsuno H, Toriyama M, Baba K, Mori T, Hakoshima T, Kanemura Y, Watanabe R, Inagaki N.
    Grip and slip of L1-CAM on adhesive substrates direct growth cone haptotaxis.
    Proc Natl Acad Sci U S A. 2018 Mar 13;115(11):2764-2769. doi: 10.1073/pnas.1711667115. Epub 2018 Feb 26. NAIST press release

  15. Maki K, Han SW, Hirano Y, Yonemura S, Hakoshima T, Adachi T.
    Real-time TIRF observation of vinculin recruitment to stretched α-catenin by AFM.
    Sci Rep. 2018 Jan 25;8(1):1575.

  16. Hakoshima T.
    Structural basis of the specific interactions of GRAS family proteins.
    FEBS Lett. 2018 Feb;592(4):489-501. doi: 10.1002/1873-3468.12987. Epub 2018 Feb 6. Review.

  17. Mori T, Ito T, Liu S, Ando H, Sakamoto S, Yamaguchi Y, Tokunaga E, Shibata N, Handa H, Hakoshima T.
    Structural basis of thalidomide enantiomer binding to cereblon.
    Sci Rep. 2018 Jan 22;8(1):1294.   ***
    Top 10 in Biochemistry and Molecular Biology

  18. Jaturontakul K, Jatuyosporn T, Laohawutthichai P, Kim SY, Mori T, Supungul P, Hakoshima T, Tassanakajon A, Krusong K.
    Molecular Characterization of Viral Responsive Protein 15 and Its Possible Role in Nuclear Export of Virus in Black Tiger Shrimp Penaeus monodon.
    Sci Rep. 2017 Jul 26;7(1):6523. doi: 10.1038/s41598-017-06653-7.

  19. Chek MF, Kim SY, Mori T, Arsad H, Samian MR, Sudesh K, Hakoshima T.
    Structure of polyhydroxyalkanoate (PHA) synthase PhaC from Chromobacterium sp. USM2, producing biodegradable plastics.
    Sci Rep. 2017 Jul 13;7(1):5312. doi: 10.1038/s41598-017-05509-4.

  20. Hirano Y, Nakagawa M, Suyama T, Murase K, Shirakawa M, Takayama S, Sun TP, Hakoshima T.
    Structure of the SHR-SCR heterodimer bound to the BIRD/IDD transcriptional factor JKD.
    Nat Plants. 2017 Feb 17;3:17010. doi: 10.1038/nplants.2017.10. NAISTプレスリリース

  21. Kim SY, Tachioka Y, Mori T, Hakoshima T.
    Structural basis for autoinhibition and its relief of MOB1 in the Hippo pathway.
    Sci Rep. 2016 Jun 23;6:28488. doi: 10.1038/srep28488.

  22. Maki K, Han SW, Hirano Y, Yonemura S, Hakoshima T, Adachi T.
    Mechano-adaptive sensory mechanism of α-catenin under tension.
    Sci Rep. 2016 Apr 25;6:24878. doi: 10.1038/srep24878.

  23. Murase K, Hirano Y, Takayama S, Hakoshima T.
    Efficient expression of SRK intracellular domain by a modeling-based protein engineering.
    Protein Expr Purif. 2017 Mar;131:70-75. doi: 10.1016/j.pep.2015.09.020. Epub 2015 Sep 21.

  24. Terawaki SI, Kitano K, Aoyama M, Mori T, Hakoshima T.
    MT1-MMP recognition by ERM proteins and its implication in CD44 shedding.
    Genes Cells. 2015 Aug 20. doi: 10.1111/gtc.12276. [Epub ahead of print]

  25. Shibahara T, Hirano Y, Hakoshima T.
    Structure of the free form of the N-terminal VH1 domain of monomeric α-catenin.
    FEBS Lett. 2015 Jul 8;589(15):1754-60.

  26. Kitano K.
    "Structural mechanisms of human RecQ helicases WRN and BLM." (Review)
    Front. Genet. 2014 Oct 29;5:366.

  27. Chamberlain, P. P., Lopez-Girona, A., Miller, K., Carmel, G., Pagarigan, B., Leon, B., Rychak, E., Corral, L., Ren, Y. J., Wang, M., Riley, M., Delker, S., Ito, T., Ando H., Mori, T., Hirano, Y., Handa, H., Hakoshima, T., Daniel, T. O. and Cathers, B. E.
    "Structure of the human Cereblon-DDB1-lenalidomide complex reveals basis for responsiveness to thalidomide analogs."
    Nat. Struct. Mol. Biol. 2014 Sept; 21 (9), 803-809. NAISTプレスリリース

  28. Mori T, Gotoh S, Shirakawa M, Hakoshima T.
    "Structural basis of DDB1-and-Cullin 4-associated Factor 1 (DCAF1) recognition by merlin/NF2 and its implication in tumorigenesis by CD44-mediated inhibition of merlin suppression of DCAF1 function."
    Genes Cells. 2014 Aug;19(8):603-19.

  29. Maesaki R, Satoh R, Taoka M, Kanaba T, Asano T, Fujita C, Fujiwara T, Ito Y, Isobe T, Hakoshima T, Maenaka K, Mishima M.
    "Efficient and cost effective production of active-form human PKB using silkworm larvae."
    Sci Rep. 2014 Aug 15;4:6016.

  30. Hakoshima, T
    "Leucine zippers (2nd version)." (Review)
    Citable Reviews in the Life Sciences (eLS 2014), John Wiley & Sons Ltd

  31. Nakagawa M, Kagiyama M, Shibata N, Hirano Y, Hakoshima T.
    "Mechanism of high-affinity abscisic acid binding to PYL9/RCAR1."
    Genes Cells.
    2014 May;19(5):386-404. doi: 10.1111/gtc.12140. Epub 2014 Mar 19..

  32. Kim SY, Hakoshima T, Kitano K.
    "Structure of the RecQ C-terminal domain of human Bloom syndrome protein."
    Sci Rep. 2013 Nov 21;3:3294.

  33. Nasuno R, Hirano Y, Itoh T, Hakoshima T, Hibi T, Takagi H.
    "Structural and functional analysis of the yeast N-acetyltransferase Mpr1 involved in oxidative stress tolerance via proline metabolism."
    Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11821-6.  NAISTプレスリリース

  34. Kagiyama M, Hirano Y, Mori T, Kim SY, Kyozuka J, Seto Y, Yamaguchi S, Hakoshima T.
    "Structures of D14 and D14L in the strigolactone and karrikin signaling pathways."
    Genes Cells. 2013 Feb;18(2):147-60.

  35. Kanaba T, Maesaki R, Mori T, Ito Y, Hakoshima T, Mishima M.
    "Microtubule-binding sites of the CH domain of EB1 and its autoinhibition revealed by NMR."
    Biochim Biophys Acta. 2013 Feb;1834(2):499-507.

  36. Hirano Y, Hatano T, Takahashi A, Toriyama M, Inagaki N, Hakoshima T.
    "Structural basis of cargo recognition by the myosin-X MyTH4–FERM domain."
    EMBO J. 2011 Jun 3;30(13):2734-47. doi: 10.1038/emboj.2011.177.

  37. Sato A, Mishima M, Nagai A, Kim SY, Ito Y, Hakoshima T, Jee JG, Kitano K.
    "Solution structure of the HRDC domain of human Bloom syndrome protein BLM."
    J Biochem. 2010 Oct;148(4):517-25.    

  38. Nishimura A, Kitano K, Takasaki J, Taniguchi M, Mizuno N, Tago K, Hakoshima T, Itoh H.
    "Structural basis for the specific inhibition of heterotrimeric Gq protein by a small molecule."
    Proc Natl Acad Sci U S A. 2010 Aug 3;107(31):13666-71.  NAISTプレスリリース   NAIST研究業績トピックス

  39. Kohno T, Yamaguchi H, Hakoshima T.
    "Crystallization and preliminary X-ray crystallographic analysis of human phosphodiesterase 12."
    Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1;66(Pt 5):520-2.

  40. Shibata N, Kagiyama M, Nakagawa M, Hirano Y, Hakoshima T.
    "Crystallization of the plant hormone receptors PYL9/RCAR1, PYL5/RCAR8 and PYR1/RCAR11 in the presence of (+)-abscisic acid."
    Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Apr 1;66(Pt 4):456-9.

  41. Kitano K, Kim SY, Hakoshima T.
    "Structural basis for DNA strand separation by the unconventional winged-helix domain of RecQ helicase WRN."
    Structure. 2010 Feb 10;18(2):177-187.     NAISTプレスリリース   NAIST研究業績トピックス

  42. Terawaki S, Kitano K, Mori T, Zhai Y, Higuchi Y, Itoh N, Watanabe T, Kaibuchi K, Hakoshima T.
    "The PHCCEx domain of Tiam1/2 is a novel protein- and membrane-binding module."
    EMBO J. 2010 Jan 6;29(1):236-50.

  43. Murase K, Hirano Y, Sun TP, Hakoshima T.
    "Gibberellin-induced DELLA recognition by the gibberellin receptor GID1."
    Nature. 2008 Nov 27;456(7221):459-463. NAISTプレスリリース   NAIST研究業績トピックス

  44. Terawaki S, Kitano K, Hakoshima T.
    "Crystallographic characterization of the membrane-targeting domains of the Rac-specific guanine nucleotide-exchange factors Tiam1 and Tiam2."
    Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Nov 1;64(Pt 11):1039-42.

  45. Terawaki S, Kitano K, Aoyama M, Hakoshima T.
    "Crystallographic characterization of the radixin FERM domain bound to the cytoplasmic tail of membrane-type 1 matrix metalloproteinase (MT1-MMP)."
    Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Oct 1;64(Pt 10):911-3.

  46. Mori T, Kitano K, Terawaki S, Maesaki R, Fukami Y, Hakoshima T.
    "Structural Basis for CD44 Recognition by ERM Proteins."
    J Biol Chem. 2008 Oct 24;283(43):29602-12.

  47. Takai Y, Kitano K, Terawaki S, Maesaki R, Hakoshima T.
    "Structural basis of the cytoplasmic tail of adhesion molecule CD43 and its binding to ERM proteins."
    J Mol Biol. 2008 Sep 5;381(3):634-44.

  48. Gohda K, Hakoshima T.
    "A molecular mechanism of P-loop pliability of Rho-kinase investigated by molecular dynamic simulation."
    J Comput Aided Mol Des. 2008 Nov;22(11):789-97. .

  49. Sakurai S, Kitano K, Morioka H, Hakoshima T.
    "Crystallization and preliminary crystallographic analysis of the catalytic domain of human flap endonuclease 1 in complex with a nicked DNA product: use of a DPCS kit for efficient protein-DNA complex crystallization."
    Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jan 1;64(Pt 1):39-43.

  50. Takai Y, Kitano K, Terawaki S, Maesaki R, Hakoshima T.
    "Structural basis of PSGL-1 binding to ERM proteins."
    Genes Cells. 2007 Dec;12(12):1329-38.

  51. Mori T, Kitano K, Terawaki S, Maesaki R, Hakoshima T.
    "Crystallographic characterization of the radixin FERM domain bound to the cytoplasmic tail of adhesion molecule CD44."
    Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Oct 1;63(Pt 10):844-7.

  52. Hakoshima T, Ichihara H.
    "Structure of SixA, a Histidine Protein Phosphatase of the ArcB Histidine-Containing Phosphotransfer Domain in Escherichia coli."
    Methods Enzymol. 2007;422:288-304.

  53. Terawaki, S., Kitano, K., Hakoshima, T.
    "Structural basis for type II membrane protein binding by ERM proteins revealed by the radixin-neutral endopeptidase 24.11 (NEP) complex."
    J Biol Chem. 2007 Jul 6;282(27):19854-62.

  54. Mishima M, Maesaki R, Kasa M, Watanabe T, Fukata M, Kaibuchi K, Hakoshima T.
    "Structural basis for tubulin recognition by cytoplasmic linker protein 170 and its autoinhibition."
    Proc Natl Acad Sci U S A. 2007 Jun 19;104(25):10346-51.

  55. Nishino TG, Kitano K, Kojima K, Ogishima T, Ito A, Kitada S.
    "Spatial orientation of mitochondrial processing peptidase and a preprotein revealed by fluorescence resonance energy transfer."
    J Biochem. 2007 Jun;141(6):889-95.

  56. Kitano K, Yoshihara N, Hakoshima T.
    "Crystal structure of the HRDC domain of human Werner syndrome protein, WRN."
    J Biol Chem. 2007 Jan 26;282(4):2717-28.    NAIST研究業績トピックス

  57. Okada K, Ichihara H, Takahashi H, Fujita N, Ishihama A, Hakoshima T.
    "Preparation and preliminary X-ray diffraction analysis of crystals of bacterial flagellar sigma factor sigma 28 in complex with the sigma 28-binding region of its antisigma factor, FlgM."
    Acta Crystallograph Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt 3):196-9.

  58. Takai Y, Kitano K, Terawaki SI, Maesaki R, Hakoshima T.
    "Crystallographic characterization of the radixin FERM domain bound to the cytoplasmic tails of adhesion molecules CD43 and PSGL-1."
    Acta Crystallograph Sect F Struct Biol Cryst Commun. 2007 Jan 1;63(Pt 1):49-51.

  59. Yamaguchi H, Miwa Y, Kasa M, Kitano K, Amano M, Kaibuchi K, Hakoshima T.
    "Structural basis for induced-fit binding of rho-kinase to the inhibitor y-27632."
    J Biochem. 2006 Sep;140(3):305-11.

  60. Furukawa N, Saito M, Hakoshima T, Kohno K.
    "A diphtheria toxin receptor deficient in epidermal growth factor-like biological activity."
    J Biochem. 2006 Dec;140(6):831-41.

  61. Yanuar A, Sakurai S, Kitano K, Hakoshima T.
    "Crystal structure of human Rad GTPase of the RGK-family."
    Genes Cells. 2006 Aug;11(8):961-8.

  62. Terawaki S, Maesaki R, Hakoshima T.
    "Structural Basis for NHERF Recognition by ERM Proteins."
    Structure. 2006 Apr;14(4):777-89.

  63. Kitano K, Yusa F, Hakoshima T.
    "Structure of dimerized radixin FERM domain suggests a novel masking motif in C-terminal residues 295-304."
    Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006 Apr 1;62(Pt 4):340-5.

  64. Yamaguchi H, Kasa M, Amano M, Kaibuchi K, Hakoshima T.
    "Molecular mechanism for the regulation of rho-kinase by dimerization and its inhibition by fasudil."
    Structure. 2006 Mar;14(3):589-600.   報道一覧 NAIST研究業績トピックス

  65. Kitano K, Kita A, Hakoshima T, Niimura Y, Miki K.
    ”Crystal structure of decameric peroxiredoxin (AhpC) from Amphibacillus xylanus”.
    Proteins. 2005;59(3):644-647.

  66. Yanuar A, Sakurai S, Kitano K, Hakoshima T.
    "Expression, purification, crystallization and preliminary crystallographic analysis of human Rad GTPase."
    Acta Crystallograph Sect F Struct Biol Cryst Commun. 2005 Nov 1;61(Pt 11):978-80.

  67. Sakurai S, Kitano K, Yamaguchi H, Hamada K, Okada K, Fukuda K, Uchida M, Ohtsuka E, Morioka H, Hakoshima T.
    "Structural basis for recruitment of human flap endonuclease 1 to PCNA."
    EMBO J. 2005 Feb 23;24(4):683-93.

  68. Hamada K, Kato M, Shimizu T, Ihara K, Mizuno T, Hakoshima T.
    "Crystal structure of the protein histidine phosphatase SixA in the multistep His-Asp phosphorelay."
    Genes Cells. 2005 Jan;10(1):1-11.

  69. Maita N, Hatakeyama K, Okada K, Hakoshima T.
    "Structural basis of biopterin-induced inhibition of GTP cyclohydrolase I by GFRP, its feedback regulatory protein."
    J Biol Chem. 2004 Dec 3;279(49):51534-40.

  70. Hakoshima T, Shimizu T, Maesaki R. (Review)
    "Structural basis of the Rho GTPase signaling."
    J Biochem. 2003 Sep;134(3):327-31.

  71. Shimizu, T., Ihara, K., Maesaki, R., Amano, M., Kaibuchi, K., Hakoshima, T. (2003).
    "Parallel coiled-coil association of the RhoA-binding domain in Rho-kinase."
    J Biol Chem, 278, 46046-46051.

  72. Sakurai, S., Kitano, K., Okada, K., Hamada, K., Morioka, H., Hakoshima, T. (2003).
    "Preparation and crystallization of human flap endonuclease FEN-1 in complex with proliferating-cell nuclear antigen, PCNA."
    Acta Crystallogr D Biol Crystallogr, 59, 933-5.

  73. Hamada, K.,Shimizu, T., Yonemura, S., Tsukita, S., and Hakoshima, T. (2003).
    "Structural basis of adhesion-molecule recognition by ERM proteins revealed by the crystal structure of the radixin-ICAM-2 complex."
    EMBO J, 22, 502-514.

  74. Terawaki, S.I., Maesaki, R., Okada, K., and Hakoshima, T. (2003).
    "Crystallographic characterization of the radixin FERM domain bound to the C-terminal region of the human Na+/H+-exchanger regulatory factor (NHERF)."
    Acta Crystallogr D Biol Crystallogr, 59, 177-179.

  75. Maita, N., Okada, K., Hatakeyama, K., and Hakoshima, T. (2002).
    "Crystalstructure of the stimulatory complex of GTP cyclohydrolase I and its feedback regulatory protein GFRP."
    Proc Natl Acad Sci U S A, 99, 1212-1217.

  76. Shimizu, T., Seto, A., Maita, N., Hamada, K., Tsukita, S., and Hakoshima, T. (2002).
    "Structural basis for neurofibromatosis type 2.Crystalstructure of the merlin FERM domain."
    J Biol Chem, 277, 10332-10336.

  77. The crystal structure of N(1)-[2-(2-amino-ethylamino)-ethyl]-ethane-1,2-diamine (polyamines) binding to the minor groove of d(CGCGCG)(2), hexamer at room temperature.
    Ohishi H1, Suzuki K, Ohtsuchi M, Hakoshima T, Rich A.
    FEBS Lett. 2002 Jul 17;523(1-3):29-34.

  78. Oka, T., Hakoshima, T., Itakura, M., Yamamori, S., Takahashi, M., Hashimoto, Y., Shiosaka, S., and Kato, K. (2002).
    "Role of loop structures of neuropsin in the activity of serine protease and regulated secretion"
    J. Biol. Chem., 277, 14724-14730.

  79. Hamada, K., Seto, A., Shimizu, T., Matsui, T., Takai, Y., Tsukita, S., and Hakoshima, T. (2001).
    "Crystallization and preliminary crystallographic studies of RhoGDI in complex with the radixin FERM domain."
    Acta Crystallogr D Biol Crystallogr, 57, 889-890.

  80. Hamada, K.,Shimizu, T., Matsui, T., Tsukita, S., and Hakoshima, T. (2001).
    "Crystallographic characterization of the radixin FERM domain bound to the cytoplasmic tail of the adhesion protein ICAM-2."
    Acta Crystallogr D Biol Crystallogr, 57, 891-892.

  81. Ishikawa, H., Tamura, A., Matsui, T., Sasaki, H., Hakoshima, T., Tsukita, Sh., and Tsukita, Sa. (2001).
    "Structural conversion between open and closed forms of radixin: low-angle shadowing electron microscopy"
    J. Mol. Biol., 310(5), 973-978.

  82. Maita, N., Okada, K., Hirotsu, S., Hatakeyama, K., and Hakoshima, T. (2001).
    "Preparation and crystallization of the stimulatory and inhibitory complexes of GTP cyclohydrolase I and its feedback regulatory protein GFRP."
    Acta Crystallogr D Biol Crystallogr, 57, 1153-1156.

  83. Hamada, K.,Shimizu, T., Matsui, T., Tsukita, S., and Hakoshima, T. (2000).
    "Structural basis of the membrane-targeting and unmasking mechanisms of the radixin FERM domain."
    EMBO J, 19, 4449-4462.

  84. Fujii, Y.,Shimizu, T., Toda, T., Yanagida, M., and Hakoshima, T. (2000).
    "Structural basis for the diversity of DNA recognition by bZIP transcription factors."
    Nat Struct Biol, 7, 889-893.

  85. Shimizu, T., Ihara, K., Maesaki, R., Kuroda, S., Kaibuchi, K., and Hakoshima, T. (2000).
    "An open conformation of switch I revealed by the crystal structure of a Mg2+-free form of RHOA complexed with GDP. Implications for the GDP/GTP exchange mechanism."
    J Biol Chem, 275, 18311-18317.

  86. Hamada, K., Matsui, T., Tsukita, Sh., Tsukita, Sa., and Hakoshima, T. (2000).
    "Crystallographic characterization of the membrane-binding domain of radixin"
    Acta Cryst. D. Biol. Crystallogr., 56(7), 922-923.

  87. Ihara, K.,Shimizu, T., Maesaki, R., Amano, M., Kaibuchi, K., and Hakoshima, T. (2000).
    "Crystallization and preliminary crystallographic analysis of the Rho-binding domain of bovine Rho-kinase"
    Acta Cryst. D. Biol. Crystallogr., 56(8), 1042-1044.

  88. Hamada, K., Kato, M., Mizuno, T., and Hakoshima, T. (1999).
    "Crystallographic characterization of a novel protein SixA that exhibits phospho-histidine phosphatase activity in the multistep His-Asp phosphorelay"
    Acta Cryst. D, Biol. Crystallogr., 55(1), 269-271.

  89. Kishi, T., Kato, M.,Shimizu, T., Kato, K., Matsumoto, K,. Yoshida, S., Shiosaka, S., and Hakoshima, T. (1999).
    "Crystalstructure of neuropsin, a hippocampal protease involved in kindling epileptogenesis"
    J. Biol. Chem., 274(7) 4220-4224.

  90. Kato, M., Mizuno, T.,Shimizu, T., and Hakoshima, T. (1999).
    "The crystal structure of the histidine containing phosphotransfer (HPt) domain of the anaerobic sensor kinase ArcB complexed with the chemotaxis response regulator CheY"
    Acta Cryst. D, Biol. Crystallogr., 55(7), 1257-1263.

  91. Kato, M., Mizuno, T.,Shimizu, T., and Hakoshima, T. (1999).
    "The refined crystal structure of the histidine-containing phosphotransfer (HPt) domain of the anaerobic sensor kinase ArcB from Escherichia coli. at 1.57 A resolution"
    Acta Cryst. D, Biol. Crystallogr., 55(11), 1842-1849.

  92. Takahashi, N., Tsukamoto, Y., Shiosaka, S., Hakoshima, T., Arata, Y., Yamaguchi, Y., Kato, K., and Shimada,I.(1999).
    "N-Glycan structure of murine hippocampus serine protease, neuropsin, produced in Trichoplusia ni cells"
    Glycoconjugate J., 16, 405-414.

  93. Hirotsu, S., Abe, Y., Iwahara, S., Hori, H., Nishino, T., Okada, K., and Hakoshima, T. (1999).
    "Crystallographic characterization of a stress-induced multifunctional protein, rat HBP23"
    J. Struct. Biol., 126 (1), 80-83.

  94. Hirotsu, S., Abe, Y., Okada, K., Nagahara, N., Hori, H., Nishino, T., and Hakoshima, T. (1999).
    "Crystalstructure of a multifunctional 2-Cys peroxiredoxin, heme-binding protein 23kDa/proliferation-associated gene product"
    Proc. Natl. Acad. Sci. USA, 96(22), 12333-12338.

  95. Fujii, Y.,Shimizu, T., Kusumoto, M., Kyougoku, Y., Taniguchi, T. and Hakoshima, T. (1999).
    "Crystal structure of an IRF/DNA complex reveals a novel DNA recognition and cooperactive binding to a tandem repeat of the core sequences"
    EMBO J., 18(18), 5028-5041.

  96. Tomita, K., Ohishi, H., Nakanishi,I., Hakoshima, T., and Rich, A. (1999).
    "Interaction between the left handed Z-DNA and polyamine -3: The Crystal structure of a left handed Z-DNA hexisamer, d(CG)3, duplex complexed with synthetic polyamine reveals binding a polyamine in the minor groove"
    Nucleosides & Nucleotides, 18(6&7), 1567-1569.

  97. Maesaki, R.,Shimizu, T., Ihara, K., Kuroda, S., Kaibuchi, K., and Hakoshima, T. (1999).
    "Biochemical and crystallographic characterization of aRhoeffector domain of the protein serine/threonine kinase N in a complex with RhoA"
    J. Struct. Biol., 126(2), 166-170.

  98. Maesaki, R., Ihara, K.,Shimizu, T., Kuroda, S., Kaibuchi, K., and Hakoshima, T. (1999).
    "The structural basis ofRhoeffector recognition revealed by theCrystalstructure of human RhoA complexed with the effector domain of of PKN/PRK1"
    Molecular Cell, 4(5), 793-803.

  99. Kato, M., Mizuno, T., and Hakoshima, T. (1998).
    "Crystallization and Preliminary X ray Analysis of the chemotaxis response regulator CheY complexed with the HPt Domain of the Anaerobic Sensor Protein ArcB"
    Acta Crystallogr. D. Biol. Crystallogr., 54 (1), 140-142.

  100. Ihara, K., Muraguchi, S., Kato, M.,Shimizu, T., Kuroda, S., Kaibuchi, K., and Hakoshima, T. (1998).
    "Crystalstructure of human RhoA in a dominantly active form complexed with GTP analogue"
    J. Biol. Chem., 273(16), 9656-9666.

  101. Kusumoto, M., Fujii, Y., Tsukuda, Y., Ohhira, T., Uegaki, K., Kyougoku, Y., Taniguchi, T., and Hakoshima, T. (1998).
    "Crystallographic characterization of the DNA binding domain of interferon regulatory factor-2 complexed with DNA"
    J. Struct. Biol., 121(3), 363-366.

  102. Fujii, Y., Ohira, T., Kyougoku, Y,. Toda, T., Yanagida, M., and Hakoshima, T. (1998).
    "Crystallographic characterization of Pap1-DNA complex"
    Acta Crystallogr. D. Biol. Crystallogr., 54 (5), 1014-1016.

  103. Ueno, A., Ohashi, H., Nakano, T., Kimura, K.,Shimizu, T., Hakoshima, T., and Iijima, H. (1998).
    "Crystalstructure of sulochrin derivatives"
    Chem. Pharm. Bull., 46(12), 1929-1931.

  104. Sugimoto, Y., Kato, M.,Shimizu, T., Hakoshima, T., and Isogai, A. (1998).
    "Dechloroacutumine from cultured roots of Menispermum Dauricum"
    Phytochemistry, 49(5), 1293-1297.

  105. Toumoto, A.,Shimizu, T.,Shimizu, M., Kyogoku, Y., Ogawa, N., Oshima, Y. and Hakoshima, T. (1997).
    "Preliminary X-ray studies on a new crystal form of PHO4-DNA complex"
    Acta Crystallogr., D53(1), 103-104.

  106. Kato, M., Mizuno, T.,Shimizu, T. and Hakoshima, T. (1997).
    "Insights into multi step phosphorelay from the crystal structure of the C-terminal HPt domain of ArcB"
    Cell, 88(5), 717-723.

  107. Kishi, T., Kato, M.,Shimizu, T., Kato, K., Matsumoto, K,. Yoshida, S., Shiosaka, S. and Hakoshima, T. (1997).
    "Crystallization and preliminary X-ray analysis of neuropsin, a serine protease expressed in the limbic system of mouse brain."
    J. Struct. Biol., 118(3), 248-251.

  108. Shimizu, T., Toumoto, A.,Shimizu, M., Kyogoku, Y., Ogawa, N., Oshima, Y. and Hakoshima, T. (1997).
    "Crystalstructure of PHO4bHLH domain-DNA complex: Flanking base recognition"
    EMBO J., 16(15), 4689-4697.

  109. Kuge, S., Fujii, Y.,Shimizu, T., Hirose, F., Matsukage, A. and Hakoshima, T. (1997).
    "Use of a fusion protein to obtain well-ordered crystals suitable for X-ray analysis: crystallization of a GST fusion protein containing the DNA-binding domain of DNA replication related element-binding factor, DREF"
    Protein Sci., 6(8), 1783-1786.

  110. Yaku, H., Kato, M., Hakoshima, T. and Mizuno, T. (1997).
    "Interaction between the CheY response regulator and the histidine-containing phosphotransfer (HPt) domain of the ArcB sensor kinase in Escherichia coli."
    FEBS Lett., 408(3), 337-340.

  111. Kato, M., Ishige, K., Mizuno, T.,Shimizu, T., and Hakoshima, T. (1996).
    "Crystallization and Preliminary X-ray Analysis of a Histidine Kinase Domain of the Anaerobic Sensor Protein ArcB from Escherichia coli"
    Acta Crystallogr D Biol Crystallogr. 1996 Nov 1;52(Pt 6):1214-5.

  112. Ohishi, H., Nakanishi, I., Inubushi, K., Van del Marel, G., Van Boom, J., Rich, A., Wang, A. H. J., Hakoshima, T., and Tomita, K. (1996).
    "Interaction between the left handed Z-DNA and polyamine -2: The crystal structure of the d(CG)3 and spermidine complex"
    FEBS Lett., 391(1-2), 153-156.

  113. Ohishi, H., Nakanishi, I., Inubushi, K., Van del Marel, G., Van Boom, J., Rich, A., Wang, A. H. J., Hakoshima, T., and Tomita, K. (1996).
    "Interaction between the left handed Z-DNA and polyamine -3: The crystal structure of the d(CG)3 and thermospermine complex"
    FEBS Lett., 398(2-3), 291-296.

    (大阪大学 Osaka university)

  114. K. Gohda, T. Itoh, Y. Hiramatsu, K. Tomita, S. Nishikawa, S. Uesugi, H. Morioka, E. Ohtsuka, M. Ikehara, and T. Hakoshima*
    "Crystal structure of RNase T1 (Y45W) complexed with 3'AMP and GflpA."
    J. Biochem. 114 (6), 842-848 (1993).

  115.  T. Hakoshima*, Y. Teranishi, T. Ohira, K. Suzuki, M. Shimizu, M. Shirakawa, Y. Kyogoku, N. Ogawa, and Y. Ohshima
    "Crystallographic characterization of PHO4/DNA complex."
    J. Mol. Biol. 229 (2), 566-569 (1993).

  116. W. -C. Lam, A. H. Maki*, T. Itoh, and T. Hakoshima
    "Phosphorescence and optically detected magnetic resonance measurements of the 2'AMP and 2'GMP complexes of a mutant ribonuclease T1 (Y45W) in solution: Correlation with X-ray crystal structures."
    Biochemistry 31 (29), 6756-6760 (1992).

  117. T. Amisaki, T. Hakoshima, K. Tomita, S. Nishikawa, S. Uesugi, M. Ikehara, S. Yoneda, and K. Kitamura
    "Molecular dynamics and free energy perturbation calculations on the mutation of tyrosine 45 to tryptophan in ribonuclease T1."
    Chem. Pharm. Bull. 40 (5), 1303-1308 (1992).

  118. T. Hakoshima, T. Itoh, K. Tomita, K. Gohda, S. Nishikawa, H. Morioka, S. Uesugi, E. Ohtsuka, and M. Ikehara
    "Three-dimensional structure of a mutant ribonuclease T1 (Y45W) complexed with non-cognizable ribonucleotide, 2'AMP, and its comparison with specific complex with 2'GMP."
    J. Mol. Biol. 223 (4), 1013-1028 (1992).

  119.  T. Hakoshima, T. Itoh, K. Tomita, S. Nishikawa, H. Morioka, S. Uesugi, E. Ohtsuka, and M. Ikehara
    "Non-cognizable ribonucleotide, 2'AMP, binds to a mutant ribonuclease T1 (Y45W) at a new base-binding site but not at the guanine-recognition site."
    FEBS Lett. 290 (2), 216-220 (1991).

  120.  M. Satoh, T. Tanaka, A. Kushiro, T. Hakoshima, and K. Tomita
    "Molecular cloning, nucleotide sequence and expression of tuf B gene encoding elongation factor Tu from Thermus thermophilus HB8."
    FEBS Lett. 288 (2), 98-100 (1991).

  121.  T. Hakoshima, S. Tanaka, T. Itoh, K. Tomita, T. Amisaki, S. Nishikawa, H. Morioka, S. Uesugi, E. Ohtsuka, and M. Ikehara
    "Hydrophobic effects on protein/nucleic acid interaction: Enhancement of substrate binding by mutating tyrosine 45 to tryptophan in ribonuclease T1."
    Protein Engineering 4 (7), 793-799 (1991).

  122. H. Ohishi, S. Kunisawa, G. van der Marel, J. H. van Boom, A. Rich, A. H. -J. Wang, K. Tomita, and T. Hakoshima
    "Interaction between the left-handed Z-DNA and polyamine: The crystal structure of the d(CG) 3 and N-(2 aminoethyl)-1,4-diaminobutane complex."
    FEBS Lett. 284 (2), 238-244 (1991).

  123. T. Itoh, K. Tomita, T. Hakoshima, H. Hiroaki, S. Uesugi, T. Amisaki, S. Nishikawa, H. Morioka, E. Ohtsuka, and M. Ikehara
    "Conformation of 2'GMP bound to a mutant ribonuclease T1 (Y45W) determined by X-ray diffraction and NMR studies."
    J. Biochem. 110 (5), 677-680 (1991).

  124. T. Hakoshima, T. Itoh, K. Tomita, S. Nishikawa, H. Morioka, S. Uesugi, E. Ohtsuka, and M. Ikehara
    "Crystallization and preliminary X-ray investigation of non-specific complexes of a mutant ribonuclease T1 (Y45W) with 2'AMP and 2'UMP."
    J. Mol. Biol. 216 (3), 497-499 (1990).

  125. S. Nishikawa, J. Adiwinata, H. Morioka, T. Fujimura, T. Tanaka, S. Uesugi, T. Hakoshima, K. Tomita, S. Nakagawa-Kosugi, and M. Ikehara
    "A thermoresistant mutant of ribonuclease T1 having three disulfide bonds."
    Protein Engineering 3 (4), 443-448 (1990).

  126. T. Hakoshima, T. Itoh, T. Higo, H. Minami, M. Tanaka, K. Goda, S. Toda, K. Oka, K. Tomita, S. Nishikawa, H. Morioka, S. Uesugi, E. Ohtsuka, J. Imura, and M. Ikehara
    "Crystallographic characterization of the mutant and wild-type ribonuclease T1 complexed with several ribonucleotides."
    J. Biochem. (Tokyo) 108 (5), 695-698 (1990).

  127. K. Tomita, T. Hakoshima, K. Inubishi, S. Kunisawa, H. Ohishi, G. A. van der Marel, J. H. van Boom, A. H. -J. Wang, and A. Rich
    "Polyamine interaction with Z-DNA."
    J. Mol. Graphics 7 (7), 71-75 (1989).

  128. T. Hakoshima, and K. Tomita
    "Crystallization and preliminary X-ray investigation reveals that tumor necrosis factor is a compact trimer furnished with 3-fold symmetry."
    J. Mol. Biol. 201 (2), 455-457 (1988).

  129. S. Nishikawa, H. Morioka, T. Kimura, Y. Ueda, T. Tanaka, S. Uesugi, T. Hakoshima, K. Tomita, E. Ohtsuka, and M. Ikehara
    "Increase in nucleolytic activity of ribonuclease T1 by substitution of tryptophan 45 for tyrosine 45."
    Eur. J. Biochem. 173 (2), 389-394 (1988).

  130. T. Hakoshima, S. Toda, S. Sugio, K. Tomita, S. Nishikawa, H. Morioka, K. Fuchimura, T. Kimura, S. Uesugi, E. Ohtsuka, and M. Ikehara
    "Conformational properties of the guanine-binding site of ribonuclease T1 inferred from X-ray structure and protein engineering."
    Protein Engineering 2 (1), 55-61 (1988).

  131. S. Nishikawa, T. Kimura, H. Morioka, S. Uesugi, T. Hakoshima, K. Tomita, E. Ohtsuka, and M. Ikehara
    "Glu 46 of ribonuclease T1 is an essential residue for recognition of guanine base."
    Biochem. Biophys. Res. Commun. 150 (1), 68-74 (1988).

  132. S. Nishikawa, H. Morioka, H. J. Kim, K. Fuchimura, T. Tanaka, S. Uesugi, T. Hakoshima, K. Tomita, E. Ohtsuka, and M. Ikehara
    "Two histidine residues are essential for ribonuclease T1 activity as is the case for ribonuclease A."
    Biochemistry 26 (26), 8620-8624 (1987).

  133. A. Fujishima, T. Hakoshima, and K. Tomita
    "Crystallographic characterization of recombinant human interleukin 2."
    J. Mol. Biol. 197 (2), 373-374 (1987).

  134. A. H. Wang, G. Ughetto, G. J. Quigley, T. Hakoshima, G. van der Marel, J. H. van Boom, and A. Rich
    "The molecular structure of a DNA-triostin A complex."
    Science 225 (4667), 1115-1121 (1984).

  135.  A. H. Wang, T. Hakoshima, G. van der Marel, J. H. van Boom, and A. Rich
    "AT base pairs are less stable than GC base pairs in Z-DNA: The crystal structure of d(m5CGTAm5CG)."
    Cell 37 (5), 321-331 (1984).

  136. K. Morishita, T. Hakoshima, T. Fujiwara, K. Tomita*, T. Kaneyasu, S. Uesugi, and M. Ikehara
    "Structure of 2'-deoxy-2'-fluoroadenosine."
    Acta Crystallogr. C 40 (3), 434-436 (1984).

  137. T. Hakoshima, T. Fukui, M. Ikehara, and K. Tomita
    "Molecular structure of a double helix that has non-Watson-Crick type base pairing formed by 2-substituted poly(A) and poly(U)."
    Pro. Nat. Acad. Sci. USA 78 (12), 7309-7313 (1981).

  138. T. Hakoshima, H. Ohmori, K. Tomita*, H. Miki, and M. Ikehara
    "The crystal and molecular structure of 2'-deoxy-2'-fluoroinosine monohydrate."
    Nucle. Acids Res. 9 (3), 711-729 (1981).

  139. I. Kitagawa, H. Shibuya, H. Fujioka, Y. Yamamoto, A. Kajiwara, K. Kitamura, A. Miyao, T. Hakoshima, and K. Tomita
    "Successive methyl migration occurring in the acid treatment of 1-epoxyethyl-1,2,2- trimethyl-cyclopentane derivative."
    Tetrahedron Letters 21, 1963-1966 (1980).

PageTop