Laboratory of Systems Neurobiology and Medicine


Publications of current projects

  1. Minegishi T, Fujikawa R, Kastian RF, Sakumura Y, Inagaki N, (2021)
    Analyses of actin dynamics, clutch coupling and traction force for growth cone advance, J. Vis. Exp. e63227. (LINK)
  2. Kastian RF, Minegishi T, Inagaki N, (2021)
    Simultaneous analyses of clutch coupling and actin polymerization in dendritic spines during chemical LTP, Star Protoc. 2, 100904.(LINK)
  3. Abe K, Baba K, Huang L, Koay TW, Okano K, Hosokawa Y, Inagaki N, (2021)
    Mechanosensitive Axon Outgrowth Mediated by L1–Laminin Clutch Interface. Biophysical Jour. 120, 17, 3566-3576. (LINK)
  4. Kastian RF, Minegishi T, Baba K, Saneyoshi T, Katsuno-Kambe H, Saranpal S, Hayashi Y, Inagaki N, (2021)
    Shootin1a-mediated actin-adhesion coupling generates force to trigger structural plasticity of dendritic spines, Cell Rep, 35, 109130. (LINK)(EurekAlert!)(NAIST HP)
  5. Minegishi T. and Inagaki N. (2020)
    Forces to drive neuronal migration steps, Front. Cell Dev. Biol, doi:10.3389/fcell.2020.00863 (LINK)
  6. Urasaki, A., Morishita, S., Naka, K., Uozumi, M., Abe, K., Huang, L., Watase, E., Nakagawa, O., Kawakami, K., Matsui, Y., Bessho, Y., and Inagaki, N.(2019)
    Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system, Sci. Rep. 9:12156 . (LINK)
  7. Huang, L., Urasaki, A. and Inagaki N. (2019)
    Rab33a and Rab33ba mediate the outgrowth of forebrain commissural axons in the zebrafish brain, Sci. Rep. 9:1799. (LINK)
  8. Minegishi T., Uesugi Y., Kaneko N., Yoshida W., Sawamoto K., Inagaki N. (2018)
    Shootin1b mediates a mechanical clutch to produce force for neuronal migration, Cell Rep 25, 624-639 (LINK)(EurekAlert!)(ScienceDaily)(Medicalxpress)
  9. Baba, K., Yoshida, W., Toriyama, M., Shimada, T., Manning, C.F., Saito, M., Kohno, K., Trimmer, J.S., Watanabe, R., and Inagaki, N. (2018)
    Gradient-reading and mechano-effector machinery for netrin-1-induced axon guidance. eLife 2018;7:e34593.(LINK)(EurekAlert!)(ScienceDaily)(MedicalXpress)(Neuroscience News)(Faculty of 1000 prime special significance)
  10. Abe, K., Katsuno, H., Toriyama, M., Baba, K., Kanemura, Y., Watanabe, R. and Inagaki, N. (2018)
    Grip and slip of L1-CAM on adhesive substrates direct growth cone haptotaxis, Proc. Natl. Acad. Sci. USA, doi:10.1073/pnas.1711667115 (LINK)(Medicalxpress)(ScienceDaily)(EurekAlert!)(AsianScientist)
  11. Inagaki, N., Katsuno, H. (2017)
    Actin Waves: Origin of Cell Polarization and Migration?, Trends in Cell Biology DOI:10.1016/j.tcb.2017.02.003 (LINK)
  12. Higashiguchi, Y., Katsuta, K., Minegishi, T., Yonemura, S., Urasaki, A., Inagaki, N. (2016)
    Identification of a shootin1 isoform expressed in peripheral tissues, Cell and Tissue Research 366, 75-87 (LINK)(Get PDF)
  13. Kubo, Y., Baba, K., Toriyama, M., Minegishi, T., Sugiura, T., Kozawa, S., Ikeda, K., and Inagaki, N. (2015)
    Shootin1-cortactin interaction mediates signal-force transduction for axon outgrowth. J Cell Biol. 210, 663-676. (LINK)
  14. Katsuno, H., Toriyama, M., Hosokawa, Y., Mizuno, K., Ikeda, K., Sakumura, Y, and Inagaki, N. (2015)
    Actin migration driven by directional assembly and disassembly of membrane anchored actin filaments, Cell Reports 12, 648-660.(LINK)(Issue Highlights)
  15. Toriyama, M., Kozawa, S., Sakumura, Y. and Inagaki, N. (2013)
    Conversion of a signal into forces for axon outgrowth through Pak1-mediated shootin1 phosphorylation, Current Biology 23, 529-534 (2013).(LINK)(Faculty of 1000 special significance)
  16. Kozawa, S., Sakumura, Y., Toriyama, M., Inagaki, N., and Ikeda, K. (2013)
    Bayesian Cell Force Estimation Considering Force Directions, Neural Process Lett, DOI 10.1007/s11063-013-9320-y
  17. Nakazawa H, Sada T, Toriyama M, Tago K, Sugiura T, Fukuda M and Inagaki N. (2012)
    Rab33a mediates anterograde vesicular transport for membrane exocytosis and axon outgrowth. J. Neurosci. 32, 12712-12725.(LINK)
  18. Hirano Y, Hatano T, Takahashi A, Toriyama M, Inagaki N, Hakoshima T.(2011)
    Structural basis of cargo recognition by the myosin-X MyTH4-FERM domain, EMBO J.30, 2734-2747.
  19. Inagaki, N.,Toriyama, M. and Sakumura, Y.(2011)
    Systems biology of symmetry-breaking during neuronal polarity formation, Dev. Neurobiol.71,584-593.(LINK)
  20. Toriyama, M., Sakumura, Y., Shimada, T., Ishii, S. and Inagaki, N.(2010)
    A diffusion-based neurite length sensing mechanism involved in neuronal symmetry-breaking, Mol.Syst.Biol. 6, 394.(LINK)
  21. Yamatani, H., Kawasaki T., Mita S., Inagaki N. Hirata T. (2010)
    Proteomics analysis of temporal changes in axonal proteins during maturation, Dev. Neurobiol, Jun;70(7):523-37.
  22. Mori T., Inagaki N., Kamiguchi H.
    , Neuronal Process Outgrowth, In Lajtha F.W., Mikoshiba, K. (eds): Handbook of Neurochemistry and Molecular Neurobiology, Springer-Verlag, 2009, pp40-44.
  23. Shimada T., Toriyama M., Uemura K., Kamiguchi H., Sugiura T., Watanabe N. Inagaki N. (2008)
    Shootin1 interacts with actin retrograde flow and L1-CAM to promote axon outgrowth., J. Cell Biol. 181, 817-829.(LINK)
  24. Mori T., Wada, T., Suzuki, T., Kubota, Y., Inagaki, N. (2007)
    Singar1, a novel RUN domain-containing protein, suppresses formation of surplus axons for neuronal polarity, J. Biol. Chem. 282, 19884-19893.(LINK)(Faculty of 1000 Exceptional)
  25. Toriyama, M., Shimada, T., Kim, K-B., Mitsuba, M., Nomura, E., Katsuta, K., Sakumura, Y., Roepstorff, P., and Inagaki, N. (2006)
    Shootin1: a protein involved in the organization of an asymmetric signal for neuronal polarization.J. Cell Biol. 175, 147-157.(LINK)
  26. Katano T., Mabuchi T., Okuda-Ashitaka E., Inagaki N., Kinumi T., Ito S. (2006)
    Proteomic identification of a novel isoform of collapsin response mediator protein (CRMP-2) in spinal nerves peripheral to dorsal root ganglia, Proteomics 8, 6085-6094.
  27. Nomura, E., Katsuta, K., Ueda, T., Toriyama, M., and Mori, T. and Inagaki, N. (2004)
    Acid-labile surfactant Improves In-solution dodecyl sulfate polyacrylamide gel protein digestion for matrix-assisted laser desorption/ionization mass spectrometric peptide mapping, J. Mass Spectrometry 39, 666-672.
  28. Inagaki, N. and Katsuta, K. (2004)
    Large gel two-dimensional electrophoresis: improving recovery of cellular proteome, Curr. Proteomics 1, 35-39.
  29. Suzuki, Y., Nakagomi, S., Namikawa, K., Kiryu-Seo, S., Inagaki, N., Kaibuchi, K., Aizawa, H., Kikuchi, K., and Kiyama, H. (2003)
    Collapsin response mediator protein-2 accelerates axon regeneration of nerve-injured moter neurons of rat, J. Neurochem. 86, 1042-1050.
  30. Oguri, T., Takahata, I., Katsuta, K., Nomura, E., Hidaka, M., and Inagaki, N. (2002)
    Proteome analysis of rat hippocampal neurons by multiple large gel two-dimensional electrophoresis, Proteomics 2, 666-672.
  31. Inagaki, N., Katsuta, K., Nomura, E., Ueda, T., Toriyama, M., and Mori, T. (2002)
    High resolution large gel two-dimensional electrophoresis for proteomics, BIOforun Int. 6, 324-325.
  32. Fukata Y., Itoh T.J., Kimura T., Menager C., Nishimura T., Shiromizu T., Watanabe H., Inagaki, N., Iwamatsu A., Hotani H., Kaibuchi K. (2002)
    CRMP-2 binds to tubulin heterodimers to promote microtubule assembly, Nature Cell Biol. 4, 583-591
  33. Inagaki, N., Chihara, K., Arimura, N., Menager, C., Kawano, Y., Matsuo, N., Nishimura, T., Amano , M., and Kaibuchi, K. (2001)
    CRMP-2 induces Axons in Cultured Hippocampal Neurons. Nature Neurosci. 4, 781-782.
  34. Canossa, M., Gartner, A., Campana, G., Inagaki, N., and Thoenen, H. (2001)
    Regulated secretion of neurotrophins by metabotropic glutamate group I (mGluRI)- and Trk-receptor activation is mediated via phospholipase C signaling pathways, EMBO J. 20, 1641-1650.
  35. Taya, S., Inagaki, N., Sengiku, H., Makino, H., Iwamatsu, A., Urakawa, I., Nagano, K., Kataoka, S., and Kaibuchi, K. (2001)
    Direct Interaction of Insulin-like growth factor-1 receptor with leukemia-associated RhoGEF, J. Cell Biol.,155, 809-819.
  36. Arimura, N., Inagaki, N., Chihara, K., Menager, C., Nakamura, N., Amano, M., Iwamatsu, A., Goshima, Y., Kaibuchi, K. (2000)
    Phosphorylation of Collapsin Response Mediator Protein-2 by Rho-kinase: Evidence for Two Separate Signaling Pathways for Growth Cone Collapse. J. Biol. Chem. 275, 23973-23980.
  37. Inagaki, N., Nishizawa, M., Arimura, N., Yamamoto, H., Takeuchi, Y., Miyamoto, E., Kaibuchi, K., Inagaki, M. (2000)
    Activation of Ca2+/calmodulin-dependent Protein Kinase II within Post-synaptic Dendritic Spines of Cultured Hippocampal Neurons. J. Biol. Chem. 275, 27165-27171.
  38. Ming, G.-i., Song, H.-j., Berninger, B., Inagaki, N., Tessier-Lavine, M., and Poo, M.-m. (1999)
    Phospholipase C-g and phosinositide 3-kinase mediate cytoplasmic signaling in nerve growth cone guidance. Neuron 23, 139-148.

Contact us

Naoyuki Inagaki, MD, PhD

Graduate School of Biological Sciences
Nara Institute of Science and Technology

Ikoma 630-0192, Japan (access map)
Tel: +81-743-72-5466
Email: ninagaki(a)