Biochemistry

• Protein purification
• Antibody production and purification
• SDS-PAGE and immunoblot
• High resolution 2D electrophoresis
  
  

  We developed a large 2DE gel (93 cm x 103 cm) to separate low abundantl proteins (Oguri et al., Proteomics 2, 666-672, 2002; Inagaki and Katsuta, Curr. Proteomics 1, 35-39, 2004).

• Sensitive MAS spectrometry (Nomura et al., J. Mass Spectrometry 39, 666-672, 2004).

Molecular biology

Cell biology

• Neuronal primary culture and gene transfection
• Immunocytochemistry and electron microscopy
• Time-laps microscopy
• Single molecule imaging
• Laser tweezers (collaboration with Dr. Tadao Sugiura)
• Traction Force misroscopy
• Atomic force microscopy (collaboration with Dr. Yoichiroh Hosokawa)
• Laser manipulation (collaboration with Dr. Yoichiroh Hosokawa)

Developmental biology

• Immunohistochemistry and in situ hybridization
• Gene targeting
• In utero electroporation

Systems biology

• Quantitative Computational modeling (collaboration with Dr. Yuichi Sakumura)