Laboratory of Systems Neurobiology and Medicine

Methods

Methods in the Lab

Biochemistry

  • Protein purification
  • Antibody production and purification
  • SDS-PAGE and immunoblot
  • High resolution 2D electrophoresis

    2d

    We developed a large 2DE gel (93 cm x 103 cm) to separate low abundantl proteins (Oguri et al., Proteomics 2, 666-672, 2002; Inagaki and Katsuta, Curr. Proteomics 1, 35-39, 2004).

  • Sensitive MAS spectrometry (Nomura et al., J. Mass Spectrometry 39, 666-672, 2004).

Molecular biology

  • Protein purification
  • Antibody production and purification
  • SDS-PAGE and immunoblot

Cell biology

  • Neuronal primary culture and gene transfection
  • Immunocytochemistry and electron microscopy
  • Time-laps microscopy
  • Single molecule imaging
  • Laser tweezers (collaboration with Dr. Tadao Sugiura)

Developmental biology

  • Analysis of tissue morphogenesis using zebrafish live imaging
  • Immunohistochemistry and in situ hybridization
  • Gene targeting (collaboration with Dr. Kenji Kohno)
  • In utero electroporation

Systems biology

  • Quantitative Computational modeling (collaboration with Dr. Yuichi Sakumura)

Contact us

Naoyuki Inagaki, MD, PhD

Graduate School of Biological Sciences
Nara Institute of Science and Technology

Ikoma 630-0192, Japan (access map)
Tel: +81-743-72-5466
Email: ninagaki(a)bs.naist.jp