トピックス 2021.10.11
論文が掲載誌の表紙を飾りました !!
Akira Nishimura, Yurie Takasaki, Shota Isogai, Yoichi Toyokawa, Ryoya Tanahashi, Hiroshi Takagi, "Role of Gln79 in feedback inhibition of the yeast γ-glutamyl kinase by proline.," Microorganisms, vol.9, no.9, pp1902, Sep. 2021, doi:10.3390/microorganisms9091902
Microorganisms, Volume 9, Issue 9 (September 2021) – 210 articles
Cover Story (view full-size image): We isolated the yeast Saccharomyces cerevisiae mutant with high levels of intracellular proline and found a missense mutation on the PRO1 gene encoding the Gln79His variant of γ-glutamyl kinase Pro1, a key enzyme that controls proline biosynthesis. Biochemical and in silico analyses of the Gln79 variants revealed that the amino acid residue at position 79 is involved in the stabilization of the proline-binding pocket in Pro1 via a hydrogen-bonding network, which plays an important role in feedback inhibition. The proposed mechanism can be applied to construct proline-accumulating yeast strains. View this paper.
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https://www.mdpi.com/2076-2607/9/9/1902
