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ストレス微生物科学研究室ストレス微生物科学研究室

過去の修士・博士論文

2017(平成29)年度修了

(修士論文)

  • 酵母の発酵力調節に関連する新規因子の解析
  • 酵母におけるN-アセチルトランスフェラーゼMpr1依存的な新規アルギニン合成制御
  • 酵母ユビキチンリガーゼRsp5のエタノールストレス応答およびエタノール発酵調節に関する研究

(博士論文)

  • The metabolic regulation mechanism and physiological role of valine in the yeast Saccharomyces cerevisiae(酵母Saccharomyces cerevisiaeにおけるバリンの代謝制御機構と生理的役割)
    N. Takpho, D. Watanabe and H. Takagi: High-level production of valine by expression of the feedback inhibition-insensitive acetohydroxyacid synthase in Saccharomyces cerevisiae. Metab. Eng., S1096-7176(17)30435-4. doi: 10.1016/j.ymben.2018.02.011 (2018).
    N. Takpho, D. Watanabe and H. Takagi: Valine biosynthesis in Saccharomyces cerevisiae is regulated by the mitochondrial branched-chain amino acid aminotransferase Bat1. Microbial Cell, in press.
  • Functional analysis of the γ-glutamyl kinase mutants in baker’s yeast (パン酵母におけるγ-グルタミルキナーゼ変異体の機能解析)
    A. Tsolmonbaatar, K. Hashida, Y. Sugimoto, D. Watanabe, S. Furukawa and H. Takagi: Isolation of baker’s yeast mutants with proline accumulation that showed enhanced tolerance to baking-associated stresses. Int. J. Food Microbiol., 238, 233-240 (2016).

2016(平成28)年度修了

(修士論文)

  • 乳酸菌との相互作用による酵母の炭素代謝改変および清酒醸造への効果に関する研究
  • 大腸菌における転写因子CRPを介した硫化水素生成経路の解析
  • 大腸菌におけるエルゴチオネインの発酵生産
  • 酵母のアルコール発酵を調節する細胞壁合成経路および新規因子に関する研究

(博士論文)

  • 出芽酵母Saccharomyces cerevisiaeにおけるチオ硫酸イオン同化経路の解析
    E. Funahashi*, K. Saiki*, K. Honda, Y. Sugiura, Y. Kawano, I. Ohtsu, D. Watanabe, Y. Wakabayashi, T. Abe, T. Nakanishi, M. Suematsu and H. Takagi: A finding of thiosulfate pathway for synthesis of organic sulfur compounds in Saccharomyces cerevisiae and an improvement of ethanol production. *These authors contributed equally to this work. J. Biosci. Bioeng., 120, 666-669 (2015).
  • Molecular mechanism of sodium acetate tolerance caused by the Thr255Ala mutation in the yeast ubiquitin ligase Rsp5p(酵母ユビキチンリガーゼRsp5のThr255Ala変異による酢酸ナトリウム耐性の分子機構に関する研究)
    A. Watcharawipas, D. Watanabe and H. Takagi: Enhanced sodium acetate tolerance in Saccharomyces cerevisiae by the Thr255Ala mutation of the ubiquitin ligase Rsp5. FEMS Yeast Res., DOI: 10.1093/femsyr/fox083 (2017).
  • 酵母に見出したフラボタンパク質Tah18依存的な一酸化窒素の合成制御機構と細胞死誘導機構
    Y. Yoshikawa*, Ryo Nasuno*, N. Kawahara, A. Nishimura, D. Watanabe and H. Takagi: Regulatory mechanism of the flavoprotein Tah18-dependent nitric oxide synthesis and cell death in yeast. *These authors contributed equally to this work. Nitric Oxide, 57, 85-91 (2016).

2015(平成27)年度修了

(修士論文)

  • 大腸菌における硫黄同化経路をモニターするためのレポーター遺伝子アッセイシステムの開発とその利用
  • 酵母ユビキチンリガーゼRsp5の基質認識ドメインがエタノールストレス応答に及ぼす影響
  • 酵母のエタノール発酵調節に関わるプロテインキナーゼRim15pおよび新規因子の解析
    D. Watanabe, Y. Zhou, A. Hirata, Y. Sugimoto, K. Takagi, T. Akao, Y. Ohya, H. Takagi, and H. Shimoi: Inhibitory role of Greatwall-like protein kinase Rim15p in alcoholic fermentation via upregulating the UDP-glucose synthesis pathway in Saccharomyces cerevisiae. Appl. Environ. Microbiol., 82, 340-351 (2016)
  • 酵母ユビキチンリガーゼRsp5のC2ドメインを介したエンドサイトーシス調節機構
    D. Watanabe, H. Murai, R. Tanahashi, K. Nakamura, T. Sasaki and H. Takagi: Cooperative and selective roles of the WW domains of the yeast Nedd4-like ubiquitin ligase Rsp5 in the recognition of the arrestin-like adaptors Bul1 and Bul2. Biochem. Biophys. Res. Commun., 463, 76-81 (2015).
  • 大腸菌rppH遺伝子のチオ硫酸リプレッションにおける機能解析とその応用
    Y. Kawano*, I. Ohtsu*, A. Tamakoshi, M. Shiroyama, A. Tsuruoka, K. Saiki, K. Takumi, G. Nonaka, T. Nakanishi, T. Hishiki, M. Suematsu and H. Takagi: Involvement of the yciW gene in L-cysteine and L-methionine metabolism in Escherichia coli. *These authors contributed equally to this work. J. Biosci. Bioeng., 119, 310-313 (2015).
  • 酵母のアルギニン代謝におけるN-アセチルトランスフェラーゼMpr1依存的な新規経路の解析
    R. Nasuno, S. Hirase, S. Norifune, D. Watanabe, and H. Takagi: Structure-based molecular design for thermostabilization of N-acetyltransferase Mpr1 involved in a novel pathway of L-arginine synthesis in yeast. J. Biochem., 159, 271-277 (2016).

(博士論文)

  • 酵母のオルガネラに存在するプロリン輸送・代謝関連因子の機能解析
    I. Nishida, D. Watanabe and H. Takagi: Putative mitochondrial α-ketoglutarate-dependent dioxygenase Fmp12 controls utilization of proline as an energy source in Saccharomyces cerevisiae. Microbial Cell, 3, 391-397 (2016).
    I. Nishida*, D. Watanabe*, A. Tsolmonbaatar, T. Kaino, I. Ohtsu and H. Takagi: Vacuolar amino acid transporters upregulated by exogenous proline and involved in cellular localization of proline in Saccharomyces cerevisiae. *These authors contributed equally to this work. J. Gen. Appl. Microbiol., 62, 132-139 (2016).
  • 酵母におけるγ-グルタミルキナーゼの機能解析
    Y. Tatehashi, D. Watanabe and H. Takagi:γ-Glutamyl kinase is involved in selective autophagy of ribosomes in Saccharomyces cerevisiae. FEBS Lett., 590, 2906-2914 (2016).
    Y. Tatehashi and H. Takagi: Characterization of g-glutamyl kinase mutants from Saccharomyces cerevisiae. J. Biosci. Bioeng., 116, 576-579 (2013).
    T. Kaino, Y. Tasaka, Y. Tatehashi and H. Takagi: Functional analysis of the C-terminal region of γ-glutamyl kinase from Saccharomyces cerevisiaeBiosci. Biotech. Biochem.76, 454-461 (2012).
  • 酵母ユビキチンリガーゼRsp5による細胞膜タンパク質の品質管理機構
    T. Shiga, N. Yoshida, Y. Shimizu, E. Suzuki, T. Sasaki, D. Watanabe and H. Takagi: Quality control of plasma membrane proteins by yeast Nedd4-like ubiquitin ligase Rsp5p under environmental stress conditions. Eukaryot. Cell13, 1191-1199 (2014).

2014(平成26)年度修了

(修士論文)

  • 酵母グリセルアルデヒド-3-リン酸脱水素酵素のニトロソ化とその機能解析
  • 酵母におけるフラボタンパク質Tah18依存的な細胞死誘導機構の解明
  • 酵母の酸化ストレス耐性に関わるN-acetyltransferase Mpr1の立体構造に基づく分子設計と構造機能相関
    R. Nasuno, S. Hirase, S. Norifune, D. Watanabe, and H. Takagi: Structure-based molecular design for thermostabilization of N-acetyltransferase Mpr1 involved in a novel pathway of L-arginine synthesis in yeast. J. Biochem., 159, 271-277 (2016).
  • 酵母ユビキチンリガーゼRsp5におけるWWドメインを介した基質認識機構の解析
    D. Watanabe, H. Murai, R. Tanahashi, K. Nakamura, T. Sasaki and H. Takagi: Cooperative and selective roles of the WW domains of the yeast Nedd4-like ubiquitin ligase Rsp5 in the recognition of the arrestin-like adaptors Bul1 and Bul2. Biochem. Biophys. Res. Commun., 463, 76-81 (2015).
  • 大腸菌における新規チオ硫酸イオン輸送体YeeEDの機能解析
    Y. Kawano*, I. Ohtsu*, A. Tamakoshi, M. Shiroyama, A. Tsuruoka, K. Saiki, K. Takumi, G. Nonaka, T. Nakanishi, T. Hishiki, M. Suematsu and H. Takagi: Involvement of the yciW gene in L-cysteine and L-methionine metabolism in Escherichia coli. *These authors contributed equally to this work. J. Biosci. Bioeng., 119, 310-313 (2015).
  • 酵母における新規チオ硫酸同化経路の発見とエタノール生産への応用
    E. Funahashi*, K. Saiki*, K. Honda, Y. Sugiura, Y. Kawano, I. Ohtsu, D. Watanabe, Y. Wakabayashi, T. Abe, T. Nakanishi, M. Suematsu, and H. Takagi: A finding of thiosulfate pathway for synthesis of organic sulfur compounds in Saccharomyces cerevisiae and an improvement of ethanol production. *These authors contributed equally to this work. J. Biosci. Bioeng., 120, 666-669 (2015).

(博士論文)

  • 超低栄養性細菌Rhodococcus erythropolis N9T-4株における炭酸固定経路の生化学的解析
    N. Yoshida*, T. Yano*, K. Kedo, T. Fujiyoshi, R. Nagai, M. Iwano, E. Taguchi, T. Nishida and H. Takagi: A unique intracellular compartment formed during the oligotrophic growth of Rhodococcus erythropolis N9T-4. *These authors contributed equally to this work. Appl. Microbiol. Biotech., 101, 331-340 (2017).
  • T. Yano*, N. Yoshida*, F. Yu, M. Wakamatsu and H. Takagi: The glyoxylate shunt is essential for CO2-requiring oligotrophic growth of Rhodococcus erythropolis N9T-4. *These authors contributed equally to this work. Appl. Microbiol. Biotech., 99, 5627-5637 (2015).
  • Isolation and functional analysis of yeast ubiquitin ligase Rsp5 variants that alleviate the toxicity of human α-synuclein(ヒトα-シヌクレインの毒性を緩和する酵母ユビキチンリガーゼRsp5変異体の単離と機能解析)
    I. Wijayanti*, D. Watanabe*, S. Oshiro and H. Takagi: Isolation and functional analysis of yeast ubiquitin ligase Rsp5 variants that alleviate the toxicity of human a-synuclein. *These authors contributed equally to this work. J. Biochem., 157, 251-260 (2015).
  • 酵母の機能未知遺伝子POG1の分子機能と生理的役割の解析
    S. Oshiro and H. Takagi: The transcriptional activator Pog1 controls cell cycle and its phosphorylated form is downregulated by the ubiquitin ligase Dma2 in Saccharomyces cerevisiaeFEMS Yeast Res.14, 1015-1027 (2014).

2013(平成25)年度修了

(修士論文)

  • 酵母におけるNedd4ファミリー型ユビキチンリガーゼRsp5のC2ドメインの機能解析
    D. Watanabe, H. Murai, R. Tanahashi, K. Nakamura, T. Sasaki and H. Takagi: Cooperative and selective roles of the WW domains of the yeast Nedd4-like ubiquitin ligase Rsp5 in the recognition of the arrestin-like adaptors Bul1 and Bul2. Biochem. Biophys. Res. Commun., 463, 76-81 (2015).
  • 酵母における銅代謝関連転写因子Mac1を介したNOによる高温ストレス耐性機構の解析
    R. Nasuno, M. Aitoku, Y. Manago, A. Nishimura, Y. Sasano and H. Takagi: Nitric oxide-mediated antioxidative mechanism in yeast through the activation of the transcription factor Mac1. PLoS One9(11), e113788 (2014).
  • 酵母ユビキチンリガーゼRsp5による新規タンパク質Bul3を介した基質認識機構の解析
  • 酵母におけるN-アセチルトランスフェラーゼMpr1依存的な新規アルギニン合成系の分子機構の解析
  • 大腸菌の硫黄代謝に関わる新規CysBレギュロンyciW遺伝子の機能解析
    Y. Kawano, I Ohtsu, G. Nonaka, A. Tamakoshi, E. Funahashi, M. Ihara and H. Takagi: Enhancement of L-cysteine production by disruption of yciW in Escherichia coliJ. Biosci. Bioeng., 119, 176-179 (2015).
    Y. Kawano, I. Ohtsu, A. Tamakoshi, M. Shiroyama, A. Tsuruoka, K. Saiki, K. Takumi, G. Nonaka, T. Nakanishi, T. Hishiki, M. Suematsu and H. Takagi: Involvement of the yciW gene in L-cysteine and L-methionine metabolism in Escherichia coli. *These authors contributed equally to this work. J. Biosci. Bioeng., 119, 310-313 (2015).
  • Rhodococcus erythropolis N9T-4株が低栄養生育時に形成するオリゴボディーの機能解析
    N. Yoshida*, T. Yano*, K. Kedo, T. Fujiyoshi, R. Nagai, M. Iwano, E. Taguchi, T. Nishida and H. Takagi: A unique intracellular compartment formed during the oligotrophic growth of Rhodococcus erythropolis N9T-4. *These authors contributed equally to this work. Appl. Microbiol. Biotech., 101, 331-340 (2017).
  • 酵母に見出したTah18タンパク質依存的なNO生成とその制御機構
    Y. Yoshikawa*, R. Nasuno*, N. Kawahara, A. Nishimura, D. Watanabe and H. Takagi: Regulatory mechanism of the flavoprotein Tah18-dependent nitric oxide synthesis and cell death in yeast. *These authors contributed equally to this work. Nitric Oxide-Biol. Chem., 57, 85-91 (2016).

(博士論文)

  • 酵母の酸化ストレス耐性に関与するN-アセチルトランスフェラーゼMpr1の構造機能解析
    R. Nasuno, Y. Hirano, T. Itoh, T. Hakoshima, T. Hibi and H. Takagi: Structural and functional analysis of the yeast N-acetyltransferase Mpr1 involved in oxidative stress tolerance via proline metabolism. Proc. Natl. Acad. Sci. USA, 110, 11821-11826 (2013).
  • 酵母のNedd4様ユビキチンリガーゼRsp5のリン酸化によるアミノ酸パーミアーゼの活性制御機構の解析
    T. Sasaki and H. Takagi: Phosphorylation of a conserved Thr357 in yeast Nedd4-like ubiquitin ligase Rsp5 is involved in downregulation of the general amino acid permease Gap1. Genes Cells, 18, 459-475 (2013).
  • 大腸菌におけるチオ硫酸イオン優先的なシステイン合成制御機構
    T. Nakatani, I. Ohtsu, G. Nonaka, N. Wiriyathanawudhiwong, S. Morigasaki and H. Takagi: Enhancement of thioredoxin/glutaredoxin-mediated l-cysteine synthesis from S-sulfocysteine increases l-cysteine production in Escherichia coli. Microb. Cell Fact., 11:62 doi:10.1186/1475-2859-11-62 (2012).

2012(平成24)年度修了

(修士論文)

  • 超低栄養性細菌Rhodococcus erythropolis N9T-4株における窒素代謝
    N. Yoshida, S. Inaba and H. Takagi: Utilization of atmospheric ammonia by an extremely oligotrophic bacterium, Rhodococcus erythropolis N9T-4. J. Biosci. Bioeng., 117, 28-32 (2014).
  • 酵母のNO生成に関与するTah18の活性制御機構の解析
    A. Nishimura, N. Kawahara and H. Takagi: The flavoprotein Tah18-dependent NO synthesis confers high-temperature stress tolerance on yeast cells. Biochem. Biophys. Res. Commun., 430, 137-143 (2013).
  • 酵母Saccharomyces cerevisiaeにおける塩基性アミノ酸の細胞毒性メカニズムの解析
    D. Watanabe, R. Kikushima, M. Aitoku, A. Nishimura, I. Ohtsu, R. Nasuno and H. Takagi: Exogenous addition of histidine reduces copper availability in the yeast Saccharomyces cerevisiae. Microbial Cell, 1, 241-246 (2014).
  • 酵母の機能未知遺伝子POG1によるリチウム耐性機構の遺伝学的解析
  • 酵母ユビキチンリガーゼRsp5によるストレス下での原形質膜タンパク質の品質管理機構
    T. Shiga, N. Yoshida, Y. Shimizu, E. Suzuki, T. Sasaki, D. Watanabe and H. Takagi: Quality control of plasma membrane proteins by yeast Nedd4-like ubiquitin ligase Rsp5p under environmental stress conditions. Eukaryot. Cell, 13, 1191-1199 (2014).
  • 酵母の酸化ストレス下におけるプロリンオキシダーゼPut1とリボソームタンパク質Grc5の機能解析
  • 酵母のユビキチンリガーゼRsp5のリン酸化/脱リン酸化に関わる酵素の探索

(博士論文)

  • Microbial production of N-acetyl cis-4-hydroxy-L-proline(N-アセチル cis-4-ヒドロキシ-L-プロリンの微生物生産)
    T. M. H. Bach, R. Hara, K. Kino, I. Ohtsu, N. Yoshida and H. Takagi: Microbial production of N-acetyl cis-4-hydroxy-L-proline by coexpression of the Rhizobium L-proline cis-4-hydroxylase and the yeast N-acetyltransferase Mpr1. Appl. Microbiol. Biotech., 97, 247-257 (2013).
    T. M. H. Bach, T. Hibi, R. Nasuno, G. Matsuo, Y. Sasano and H. Takagi: Production of N-acetyl cis-4-hydroxy-L-proline by the yeast N-acetyltransferase Mpr1. J. Biosci. Bioeng., 114, 160-165 (2012).

2011(平成23)年度修了

(修士論文)

  • 酵母におけるプロリン合成の鍵酵素γ-グルタミルキナーゼの機能解析
    T. Kaino, Y. Tasaka, Y. Tatehashi and H. Takagi: Functional analysis of the C-terminal region of γ-glutamyl kinase from Saccharomyces cerevisiae. Biosci. Biotech. Biochem., 76, 454-461 (2012).
  • 酵母における一酸化窒素の下流経路の解析
    R. Nasuno, M. Aitoku, Y. Manago, A. Nishimura, Y. Sasano and H. Takagi: Nitric oxide-mediated antioxidative mechanism in yeast through the activation of the transcription factor Mac1. PLoS One, 9(11), e113788 (2014).
  • 大腸菌のシステイン/シスチンシャトルシステムにおけるシスチン取り込み機構の解析
    I. Ohtsu, Y. Kawano, M. Suzuki, S. Morigasaki, K. Saiki, S. Yamazaki, G. Nonaka and H. Takagi: Uptake of l-cystine via an ABC transporter contributes defense of oxidative stress in the l-cysteine export-dependent manner in Escherichia coli. PLoS One, 10(4), e0120619 (2015).
  • 超低栄養性細菌に見出した新規な細胞内構造体(オリゴボディー)の解析
    N. Yoshida*, T. Yano*, K. Kedo, T. Fujiyoshi, R. Nagai, M. Iwano, E. Taguchi, T. Nishida and H. Takagi: A unique intracellular compartment formed during the oligotrophic growth of Rhodococcus erythropolis N9T-4. *These authors contributed equally to this work. Appl. Microbiol. Biotech., 101, 331-340 (2017).


(博士論文)

  • 酵母におけるアルギニン/一酸化窒素の合成を介した新規抗酸化機構
    A. Nishimura, T. Kotani, Y. Sasano and H. Takagi: An antioxidative mechanism mediated by the yeast N-acetyltransferase Mpr1: Oxidative stress-induced arginine synthesis and its physiological role. FEMS Yeast Res., 10, 687-698 (2010).

2010(平成22)年度修了

(修士論文)

  • 高温ストレスに応答した酵母プロリンオキシダ―ゼ遺伝子の転写活性化機構の解析
  • 酵母におけるプロリン代謝を介した細胞保護機能
  • 酵母ユビキチンリガーゼRsp5によるエタノールストレス下での原形質膜タンパク質分解機構の解析
    T. Shiga, N. Yoshida, Y. Shimizu, E. Suzuki, T. Sasaki, D. Watanabe and H. Takagi: Quality control of plasma membrane proteins by yeast Nedd4-like ubiquitin ligase Rsp5p under environmental stress conditions. Eukaryot. Cell, 13, 1191-1199 (2014).
  • 分裂酵母を用いたtarget of rapamycin complex2の活性制御機構の解析
  • システインによる大腸菌の生育阻害機構の解析
  • 低エネルギー型炭酸固定系を有するRhodococcus erythropolis N9T-4株のC2代謝に関する研究

2009(平成21)年度修了

(修士論文)

  • 酵母ユビキチンリガーゼRsp5変異株のストレス感受性を多コピーで相補する転写因子Pog1の機能解析
  • 酵母の酸化ストレス耐性に関与するN-アセチルトランスフェラーゼMpr1の機能改変
  • 出芽酵母のオルガネラに存在する新規プロリントランスポーターの探索
  • 酸化ストレス下での解糖系酵素グリセルアルデヒド-3-リン酸脱水素酵素の機能解析と有用酵母育種への応用
  • 大腸菌における新規システイン生合成経路の探索と発酵生産への応用
    I. Ohtsu*, N. Wiriyathanawudhiwong*, S. Morigasaki, T. Nakatani, H. Kadokura and H. Takagi: The L-cysteine/L-cystine shuttle system provides reducing equivalents to the periplasm in Escherichia coli. *These authors contributed equally to this work. J. Biol. Chem., 285, 17479-17487 (2010).
  • Rhodococcus erythropolis N9T-4株におけるメタノール代謝関連酵素遺伝子の発現解析
    N. Yoshida, T. Hayasaki and H. Takagi: Gene expression analysis of methylotrophic oxidoreductases involved in the oligotrophic growth of Rhodococcus erythropolis N9T-4. Biosci. Biotech. Biochem., 75, 123-127 (2011).

(博士論文)

  • Functional analysis of L-cysteine transporters and their applications to fermentative production of L-cysteine in Escherichia coli. 
    N. Wiriyathanawudhiwong*, I. Ohtsu*, Z.-D. Li, H. Mori and H. Takagi: The outer membrane TolC is involved in cysteine tolerance and overproduction in Escherichia coli. *These authors contributed equally to this work. Appl. Microbiol. Biotech., 81, 903-913 (2009).
    I. Ohtsu*, N. Wiriyathanawudhiwong*, S. Morigasaki, T. Nakatani, H. Kadokura and H. Takagi: The L-cysteine/L-cystine shuttle system provides reducing equivalents to the periplasm in Escherichia coli. *These authors contributed equally to this work. J. Biol. Chem., 285, 17479-17487 (2010).

2008(平成20)年度修了

(修士論文)

  • 酵母のHECT型ユビキチンリガーゼRsp5を介したプロリンパーミアーゼ活性制御機構の解析
    Y. Haitani, M. Nakata, T. Sasaki, A. Uchida and H. Takagi: Engineering of the yeast ubiquitin ligase Rsp5: isolation of a new variant that induces constitutive inactivation of the general amino acid permease Gap1. FEMS Yeast Res., 9, 73-86 (2009).
  • 酵母におけるプロリンの抗酸化機能:アポトーシス様細胞死との関連性
    H. Takagi, J. Taguchi and T. Kaino: Proline accumulation protects Saccharomyces cerevisiae cells in the stationary phase from ethanol stress by reducing reactive oxygen species levels. Yeast, 33, 355-363 (2016).
  • 酵母におけるプロリン合成の鍵酵素γ-グルタミルキナーゼのドメイン構造と酵素機能の解析
    T. Kaino, Y. Tasaka, Y. Tatehashi and H. Takagi: Functional analysis of the C-terminal region of γ-glutamyl kinase fromSaccharomyces cerevisiaeBiosci. Biotech. Biochem., in press.
  • 酵母N-アセチルトランスフェラーゼMpr1による抗酸化機構の解明
    A. Nishimura, T. Kotani, Y. Sasano and H. Takagi: An antioxidative mechanism mediated by the yeast  N-acetyltransferase Mpr1: Oxidative stress-induced arginine synthesis and its physiological role.  FEMS Yeast Res., 10, 687-698 (2010).
  • 酵母N-アセチルトランスフェラーゼMpr1の機能解析:マイクロアレイデータに基づく新規生理機能の探索
  • 大腸菌外膜タンパク質TolCの機能解析とシステイン発酵生産への応用
    N. Wiriyathanawudhiwong*, I. Ohtsu*, Z.-D. Li, H. Mori and H. Takagi: The outer membrane TolC is involved in cysteine tolerance and overproduction in Escherichia coli. *These authors contributed equally to this work. Appl. Microbiol. Biotech., 81, 903-913 (2009).

(博士論文)

  • 赤色酵母 Xanthophyllomyces dendrorhous におけるアスタキサンチン高生産変異株の効率的スクリーニング法の開発と高生産機構の解析
    K. Ukibe, T. Katsuragi, Y. Tani and H. Takagi: Efficient screening for astaxanthin-overproducing mutants of the yeastXanthophyllomyces dendrorhous by flow cytometry. FEMS Microbiol. Lett., 286, 241-248 (2008).
    K. Ukibe, K. Hashida, N. Yoshida and H. Takagi: Metabolic engineering of Saccharomyces cerevisiae for astaxanthin production and oxidative stress tolerance. Appl. Environ. Microbiol., 75, 7205-7211 (2009).

2007(平成19)年度修了

(修士論文)

  • ストレス耐性の向上に関与する高機能型酵母ユビキチンリガーゼRsp5の取得と機能解析
    Y. Haitani, M. Nakata, T. Sasaki, A. Uchida and H. Takagi: Engineering of the yeast ubiquitin ligase Rsp5: isolation of a new variant that induces constitutive inactivation of the general amino acid permease Gap1. FEMS Yeast Res., 9, 73-86 (2009).
  • ランダム変異導入による酵母N-アセチルトランスフェラーゼMpr1の抗酸化能向上
    K. Iinoya, T. Kotani, Y. Sasano and H. Takagi: Engineering of the yeast antioxidant enzyme Mpr1 for enhanced activity and stability. Biotechnol. Bioeng., 103, 341-352 (2009).
  • ストレス下における酵母ユビキチンリガーゼRsp5の機能解析:基質タンパク質のユビキチン化機構の解析
    H. Hiraishi, M. Okada, I. Ohtsu and H. Takagi: Functional analysis of the yeast ubiquitin ligase Rsp5: Involvement of the ubiquitin-conjugating enzyme Ubc4 and poly-ubiquitination in ethanol-Induced down-regulation of targeted proteins.Biosci. Biotech. Biochem., 73, 2268-2273 (2009).
    T. Kaino, T. Tateiwa, S. Mizukami-Murata, J. Shima and H. Takagi: Self-cloning baker's yeasts that accumulate proline enhance freeze tolerance in doughs. Appl. Environ. Microbiol., 74, 5845-5849 (2008).
  • 酵母ユビキチンリガーゼRsp5変異株のストレス感受性を相補する遺伝子の探索
  • Fusarium属由来新規フルクトシルペプチドオキシダーゼの酵素的性質
  • 酵母Saccharomyces cerevisiaeへのアスタキサンチン合成系酵素遺伝子の導入とアスタキサンチン生産
    K. Ukibe, K. Hashida, N. Yoshida and H. Takagi: Metabolic engineering of Saccharomyces cerevisiae for astaxanthin production and oxidative stress tolerance. Appl. Environ. Microbiol., 75, 7205-7211 (2009).

(博士論文)

  • ストレスにおける酵母ユビキチンリガーゼRsp5の機能解析
    Y. Haitani, H. Shimoi and H. Takagi: Rsp5 regulates expression of stress proteins via post-translational modification of Hsf1 and Msn4 in Saccharomyces cerevisiaeFEBS Lett., 580, 3433-3438 (2006).
    Y. Haitani and H. Takagi: Rsp5 is required for the nuclear export of mRNA of HSF1 and MSN2/4 under stress conditions in Saccharomyces cerevisiaeGenes to Cells, 13, 105-116 (2008).
    Y. Haitani, M. Nakata, T. Sasaki, A. Uchida and H. Takagi: Engineering of the yeast ubiquitin ligase Rsp5: isolation of a new variant that induces constitutive inactivation of the general amino acid permease Gap1. FEMS Yeast Res., 9, 73-86 (2009).
  • 超低栄養細菌Rhodococcus erythropolis N9T-4株のCO2要求性に関する研究
    N. Ohhata, N. Yoshida, H. Egami, T. Katsuragi, Y. Tani and H. Takagi: An extremely oligotrophic bacterium,Rhodococcus erythropolis N9T-4 isolated from crude oil. J. Bacteriol., 189, 6824-6831 (2007).
    N. Yoshida, N. Ohhata, Y. Yoshino, T. Katsuragi, Y. Tani and H. Takagi: Screening of carbon dioxide-requiring extreme oligotrophs from soil. Biosci. Biotech. Biochem., 71, 2830-2832 (2007).

2006(平成18)年度修了

(修士論文)

  • 独立栄養的に生育するRhodococcus erythropolis N9T-4株のプロテオーム解析
    N. Ohhata, N. Yoshida, H. Egami, T. Katsuragi, Y. Tani and H. Takagi: An extremely oligotrophic bacterium,Rhodococcus erythropolis N9T-4 isolated from crude oil. J. Bacteriol., 189, 6824-6831 (2007).
  • フルクトシルアミノ酸オキシダーゼの細胞内局在性
  • 酵母Phaffia rhodozymaにおけるアスタキサンチン高生産メカニズムの解析
  • 遺伝子組換え大腸菌を用いた(S)-N-benzyl-3-pyrrolidinolの生産
    K. Yamada-Onodera, K. Kojima, Y. Takase and Y. Tani: Cloning, sequence analysis, and expression in  Escherichia coliof gene encoding N-benzyl-3-pyrrolidinol dehydrogenase from Geotrichum capitatumJ. Biosci. Bioeng., 104, 379-384 (2007).
  • 微生物による光学活性2-amino-1-phenylethanolの生産
    K. Yamada-Onodera, Y. Takase and Y. Tani: Purification and characterization of 2-aminoacetophenone reductase of newly isolated Burkholderia sp.YT. J. Biosci. Bioeng., 104, 416-419 (2007).
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