People
Assist. Prof.
Ryoji Miyazaki
- Hometown:Osaka, Japan
- Research Field : in vivo photo-crosslinking analysis for dynamic behaviors of cellular proteins
- One of my favorites : Hamburgers
- Special Skill: Crosslinking experiments
- Comment: I would like to spread the in vivo photo-crosslinking approach.
Education
- 2007. Apr. – 2011. Mar. Kyoto University
- 2011. Apr. – 2013. Mar. Graduate School of Science, Kyoto University, Master course
- 2013. Apr. – 2018. Mar. Graduate School of Science, Kyoto University, Doctor course
- 2018. Mar. Ph.D. in science
Research career
- 2015. Apr. - 2017. Mar. JSPS Research Fellow (DC2)
- 2019. Apr. - 2021. Feb. Postdoctoral Fellow, Institute for Frontier Life and Medical Sciences, Kyoto University University
- 2021. Mar. - Present Assistant professor, Structural Life Science Lab, Department of Systems Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology
Publications
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ºIkei M, ºMiyazaki R, Monden K, Naito Y, Takeuchi A, Takahashi YS, Tanaka Y, Mori T, *Ichikawa M, *Tsukazaki T. YeeD is an essential partner for YeeE-mediated thiosulfate uptake in bacteria and regulates thiosulfate ion decomposition. PLoS Biol. 22, e3002601 (2024) (ººcontributed equally)
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*Kohga H, Lertpreedakorn N, Miyazaki R and *Tsukazaki T. Critical residues of the antibiotic peptide LysM that inhibits lipid II flipping. bioRxiv 2023.05.22.541694 (2023)
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*Miyazaki R and *Akiyama Y. Analyzing protein intermediate interactions in living E. coli cells using site-specific photo-crosslinking combined with chemical crosslinking. STAR Protocols 4, 102178 (2023)
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Miyazaki R, Ai M, Tanaka N, Suzuki T, Dhomae N, Tsukazaki T, Akiyama Y and *Mori H. Inner membrane YfgM-PpiD heterodimer acts as a functional unit that associates with the SecY/E/G translocon and promotes protein translocation. J. Biol. Chem. 298, 102572 (2022)
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Miyazaki R, Watanabe T, Yoshitani K, *Akiyama Y. Edge-strand of BepA interacts with immature LptD on the β-barrel assembly machine to direct it to on- and off-pathways. eLife 10, e70541 (2021)
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Miyazaki R, Akiyama Y and *Mori H. Fine interaction profiling of VemP and mechanisms responsible for its translocation-coupled arrest-cancelation. eLife, 9, e62623 (2020).
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Daimon Y, Narita S, Miyazaki R, Hizukuri Y, Mori H, Tanaka Y, Tsukazaki T and *Akiyama Y. Reversible auto-inhibitory regulation of Escherichia coli metallopeptidase BepA for selective β-barrel protein degradation. Proc. Natl. Acad. Sci. USA 117, 27989-27996 (2020).
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*Yura T, Miyazaki R, Fujiwara K, Ito K, Chiba S, Mori H and *Akiyama Y. Heat shock transcription factor σ32 defective in membrane transport can be suppressed by transposon insertion into the genes for a restriction enzyme subunit or a putative autotransporter in E. coli. Genes Genet. Syst. 93, 229–235 (2018).
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Miyazaki R, Myougo N, Mori H and *Akiyama Y. A photo-cross-linking approach to monitor folding and assembly of newly synthesized proteins in a living cell. J. Biol. Chem. 293, 677-686 (2018).
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Daimon Y, Iwama-Masui C, Tanaka Y, Shiota T, Suzuki T, Miyazaki R, Sakurada H, Lithgow T, Dohmae N, Mori H, *Tsukazaki T, *Narita S and *Akiyama Y. The tetratricopeptide repeat (TPR) domain of BepA is required for productive interaction with substrate proteins and the β-barrel assembly machinery (BAM) complex. Mol. Microbiol. 106, 760-776 (2017).
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Miyazaki R, Yura T, Suzuki T, Dohmae N, Mori M and *Akiyama Y. A novel SRP recognition sequence in the homeostatic control region of heat shock transcription factor σ32. Sci. Rep. 6, 24147 (2016).
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Lim B, Miyazaki R, Neher S, Siegele DA, Ito K, Walter P, Akiyama Y, *Yura T and *Gross CA. Heat shock transcription factor σ32 co-opts the signal recognition particle to regulate protein homeostasis in E. coli. PLoS Biology, 11, e1001735 (2013).
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*Endo M, Miyazaki R, Emura T, Hidaka K and *Sugiyama H. Transcription regulation system mediated by mechanical operation of a DNA nanostructure. J. Am. Chem. Soc. 134, 2852-2855 (2012).
Reviews and Books
- 宮﨑亮次, 森博幸, 秋山芳展. PiXie法による細胞内タンパク質の迅速な相互作用・フォールディング解析. 生物物理, 61, 036–039 (2021).
- Miyazaki R, Akiyama Y and *Mori H. A photo-cross-linking approach to monitor protein dynamics in living cells. Biochim. Biopys. Acta Gen. Subj. 1864, 129317 (2020).
Awards and honors
- 2013-Sep. 第86回 日本生化学会大会 鈴木紘一メモリアル賞
- 2014-Jun. 第11回 21世紀大腸菌研究会 口頭発表大賞
- 2016-May. 「新生鎖の生物学」 第3回若手ワークショップ 最優秀学生賞
- 2017-Aug. 「新生鎖の生物学」 第4回若手ワークショップ 優秀学生賞
- 2017-Nov. 「新生鎖の生物学」 平成29年度 第1回班会議 若手ポスター最優秀賞