People

ºIkei M, ºMiyazaki R, Monden K, Naito Y, Takeuchi A, Takahashi YS, Tanaka Y, Mori T, *Ichikawa M, *Tsukazaki T.  YeeD is an essential partner for YeeE-mediated thiosulfate uptake in bacteria and regulates thiosulfate ion decomposition. PLoS Biol.   22, e3002601 (2024) (ººcontributed equally）

*Kohga H, Lertpreedakorn N, Miyazaki R and *Tsukazaki T.  Critical residues of the antibiotic peptide LysM that inhibits lipid II flipping.  bioRxiv  2023.05.22.541694 (2023)

*Miyazaki R and *Akiyama Y. Analyzing protein intermediate interactions in living E. coli cells using site-specific photo-crosslinking combined with chemical crosslinking. STAR Protocols 4, 102178 (2023)

Miyazaki R, Ai M, Tanaka N, Suzuki T, Dhomae N, Tsukazaki T, Akiyama Y and *Mori H.  Inner membrane YfgM-PpiD heterodimer acts as a functional unit that associates with the SecY/E/G translocon and promotes protein translocation.  J. Biol. Chem. 298, 102572 (2022)

Miyazaki R, Watanabe T, Yoshitani K, *Akiyama Y.  Edge-strand of BepA interacts with immature LptD on the β-barrel assembly machine to direct it to on- and off-pathways.  eLife 10, e70541 (2021)

Miyazaki R, Akiyama Y and *Mori H. Fine interaction profiling of VemP and mechanisms responsible for its translocation-coupled arrest-cancelation. eLife, 9, e62623 (2020).

Daimon Y, Narita S, Miyazaki R, Hizukuri Y, Mori H, Tanaka Y, Tsukazaki T and *Akiyama Y. Reversible auto-inhibitory regulation of Escherichia coli metallopeptidase BepA for selective β-barrel protein degradation. Proc. Natl. Acad. Sci. USA 117, 27989-27996 (2020).

*Yura T, Miyazaki R, Fujiwara K, Ito K, Chiba S, Mori H and *Akiyama Y. Heat shock transcription factor σ32 defective in membrane transport can be suppressed by transposon insertion into the genes for a restriction enzyme subunit or a putative autotransporter in E. coli. Genes Genet. Syst. 93, 229–235 (2018).

Miyazaki R, Myougo N, Mori H and *Akiyama Y. A photo-cross-linking approach to monitor folding and assembly of newly synthesized proteins in a living cell. J. Biol. Chem. 293, 677-686 (2018).

Daimon Y, Iwama-Masui C, Tanaka Y, Shiota T, Suzuki T, Miyazaki R, Sakurada H, Lithgow T, Dohmae N, Mori H, *Tsukazaki T, *Narita S and *Akiyama Y. The tetratricopeptide repeat (TPR) domain of BepA is required for productive interaction with substrate proteins and the β-barrel assembly machinery (BAM) complex. Mol. Microbiol. 106, 760-776 (2017). 

Miyazaki R, Yura T, Suzuki T, Dohmae N, Mori M and *Akiyama Y. A novel SRP recognition sequence in the homeostatic control region of heat shock transcription factor σ32. Sci. Rep. 6, 24147 (2016).

Lim B, Miyazaki R, Neher S, Siegele DA, Ito K, Walter P, Akiyama Y, *Yura T and *Gross CA. Heat shock transcription factor σ32 co-opts the signal recognition particle to regulate protein homeostasis in E. coli. PLoS Biology, 11, e1001735 (2013).

*Endo M, Miyazaki R, Emura T, Hidaka K and *Sugiyama H. Transcription regulation system mediated by mechanical operation of a DNA nanostructure. J. Am. Chem. Soc. 134, 2852-2855 (2012).