Stem Cell Technologies Lab

発表論文 / Publications


  • Activin E enhances insulin sensitivity and thermogenesis by activating brown/beige adipocytes.
    Sekiyama K, Ushiro Y, Kurisaki A, Funabe M, Hashimoto O.
    J Vet Med Sci. 2019 Mar 15.



  • Structural basis for receptor-regulated SMAD recognition by MAN1.
    Miyazono KI, Ohno Y, Wada H, Ito T, Fukatsu Y, Kurisaki A, Asashima M, Tanokura M.
    Nucleic Acids Res. 2018, 46(22):12139-12153.
  • Cardiac differentiation at an initial low density of human-induced pluripotent stem cells.
    Le MNT, Takahi M, Maruyama K, Kurisaki A, Ohnuma K.
    In Vitro Cell Dev Biol Anim. 2018, 54(7):513-522.
  • Hydrophobic patches on SMAD2 and SMAD3 determine selective binding to cofactors.
    Miyazono KI, Moriwaki S, Ito T, Kurisaki A, Asashima M, Tanokura M.
    Sci Signal. 2018, 11(523). pii: eaao7227.
  • Quartz-Seq2: a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads.
    *Sasagawa Y, *Danno H, *Takada H (* co-first), Ebisawa M, Tanaka K, Hayashi T, Kurisaki A, Nikaido I.
    Genome Biol. 2018, 19(1):29.
  • Therapeutic effects of a recombinant human collagen peptide bioscaffold with human adipose-derived stem cells on impaired wound healing after radiotherapy.
    Mashiko T, Takada H, Wu SH, Kanayama K, Feng J, Tashiro K, Asahi R, Sunaga A, Hoshi K, Kurisaki A, Takato T, Yoshimura K.
    J Tissue Eng Regen Med. 2018,  12(5):1186-1194.


  • Simple and effective generation of transgene-free induced pluripotent stem cells using an auto-erasable Sendai virus vector responding to microRNA-302.
    Nishimura K, Ohtaka M, Takada H, Kurisaki A, Tran NVK, Tran YTH, Hisatake K, Sano M, Nakanishi M.
    Stem Cell Res. 2017, 23:13-19.
  • Formation of Stomach Tissue by Organoid Culture Using Mouse Embryonic Stem Cells.
    Noguchi TK, Kurisaki A. Methods Mol Biol. 2017;1597:217-228.
  • A Role for KLF4 in Promoting the Metabolic Shift via TCL1 during Induced Pluripotent Stem Cell Generation.
    Nishimura K, Aizawa S, Nugroho FL, Shiomitsu E, Tran YTH, Bui PL, Borisova E, Sakuragi Y, Takada H, Kurisaki A, Hayashi Y, Fukuda A, Nakanishi M, Hisatake K.
    Stem Cell Reports. 2017, 8(3):787-801.


  • Ground-based assessment of JAXA mouse habitat cage unit by mouse phenotypic studies.
    Shimbo M, Kudo T, Hamada M, Jeon H, Imamura Y, Asano K, Okada R, Tsunakawa Y, Mizuno S, Yagami K, Ishikawa C, Li H, Shiga T, Ishida J, Hamada J, Murata K, Ishimaru T, Hashimoto M, Fukamizu A, Yamane M, Ikawa M, Morita H, Shinohara M, Asahara H, Akiyama T, Akiyama N, Sasanuma H, Yoshida N, Zhou R, Wang YY, Ito T, Kokubu Y, Noguchi TA, Ishimine H, Kurisaki A, Shiba D, Mizuno H, Shirakawa M, Ito N, Takeda S, Takahashi S.
    Exp Anim. 2016, 65(2):175-87.
  • Lipase member H frequently overexpressed in human esophageal adenocarcinomas.
    Ishimine H, Zhou R, Sumitomo K, Ito Y, Seki Y, Yoshida Y, Kurisaki A.
    Tumour Biol. 2016 Feb;37(2):2075-81.


  • Therapeutic Potential of Human Adipose-Derived Stem/Stromal Cell Microspheroids Prepared by Three-Dimensional Culture in Non-Cross-Linked Hyaluronic Acid Gel.
    Mineda K, Feng J, Ishimine H, Takada H, Doi K, Kuno S, Kinoshita K, Kanayama K, Kato H, Mashiko T, Hashimoto I, Nakanishi H, Kurisaki A, Yoshimura K.
    Stem Cells Transl Med. 2015, 4(12):1511-22.
  • Identification of novel proteins differentially expressed in pluripotent embryonic stem cells and differentiated cells.
    Enomoto K, Watanabe-Susaki K, Kowno M, Takada H, Intoh A, Yamanaka Y, Hirano H, Sugino H, Asashima M, Kurisaki A.
    J Med Invest. 2015, 62(3-4):130-6.
  • Emerging roles of nucleolar and ribosomal proteins in cancer, development, and aging.
    Takada H, Kurisaki A. Cell Mol Life Sci. 2015 Nov;72(21):4015-25.
  • Generation of stomach tissue from mouse embryonic stem cells.
    Noguchi TK, Ninomiya N, Sekine M, Komazaki S, Wang PC, Asashima M, Kurisaki A.
    Nature Cell Biology 2015, 17(8):984-993.
  • Establishment and culture optimization of a new type of pituitary immortalized cell line.
    Kokubu Y, Asashima M, Kurisaki A.
    Biochem. Biophys. Res. Commun.2015, 463(4):1218-1224.
  • Therapeutic Potential of Adipose-Derived SSEA-3-Positive Muse Cells for Treating Diabetic Skin Ulcers.
    Kinoshita K, Kuno S, Ishimine H, Aoi N, Mineda K, Kato H, Doi K, Kanayama K, Feng J, Mashiko T, Kurisaki A, Yoshimura K.
    Stem Cells Transl. Med. 2015, 4(2):146-155.
  • Aminolevulinate synthase 2 mediates erythrocyte differentiation by regulating larval globin expression during Xenopus primary hematopoiesis.
    Ogawa-Otomo A, Kurisaki A, Ito Y.
    Biochem. Biophys. Res. Commun. 2015, 456(1):476-481.


  • Methylome, transcriptome, and PPAR(γ) cistrome analyses reveal two epigenetic transitions in fat cells.
    Takada H, Saito Y, Mituyama T, Wei Z, Yoshihara E, Jacinto S, Downes M, Evans RM, Kida YS.
    Epigenetics. 2014 Sep;9(9):1195-206.
  • Cell and Tissue Damage after Skin Exposure to Ionizing Radiation: Short- and Long-Term Effects after a Single and Fractional Doses.
    Kinoshita K, Ishimine H, Shiraishi K, Kato H, Doi K, Kuno S, Kanayama K, Mineda K, Mashiko T, Feng J, Nakagawa K, Kurisaki A, Itami S, Yoshimura K.
    Cells Tissues Organs. 2014;200(3-4):240-52.
  • Biosynthesis of ribosomal RNA in nucleoli regulates pluripotency and differentiation ability of pluripotent stem cells.
    Watanabe-Susaki K, Takada H, Enomoto K, Miwata K, Ishimine H, Intoh A, Ohtaka M, Nakanishi M, Sugino H, Asashima M, Kurisaki A.
    Stem Cells. 2014, 32(12):3099-3111.
  • Prohibitin 2 regulates the proliferation and lineage-specific differentiation of mouse embryonic stem cells in mitochondria.
    Kowno M, Watanabe-Susaki K, Ishimine H, Komazaki S, Enomoto K, Seki Y, Wang YY, Ishigaki Y, Ninomiya N, Noguchi TA, Kokubu Y, Ohnishi K, Nakajima Y, Kato K, Intoh A, Takada H, Yamakawa N, Wang PC, Asashima M, Kurisaki A.
    PLoS One. 2014,99(4):e81552.
  • Lipase member H is a novel secreted protein selectively upregulated in human lung adenocarcinomas and bronchioloalveolar carcinomas.
    Seki Y, Yoshida Y, Ishimine H, Shinozaki-Ushiku A, Ito Y, Sumitomo K, Nakajima J, Fukayama M, Michiue T, Asashima M, Kurisaki A.
    Biochem. Biophys. Res. Commun. 2014, 443(4):1141-1147.
  • Microfluidic perfusion culture of human induced pluripotent stem cells under fully defined culture conditions.
    Yoshimitsu R, Hattori K, Sugiura S, Kondo Y, Yamada R, Tachikawa S, Satoh T, Kurisaki A, Ohnuma K, Asashima M, Kanamori T.
    Biotechnol. Bioeng. 2014, 111(5):937-947.


  • N-Cadherin is a prospective cell surface marker of human mesenchymal stem cells that have high ability for cardiomyocyte differentiation.
    Ishimine H, Yamakawa N, Sasao M, Tadokoro M, Kami D, Komazaki S, Tokuhara M, Takada H, Ito Y, Kuno S, Yoshimura K, Umezawa A, Ohgushi H, Asashima M, Kurisaki A.
    Biochem. Biophys. Res. Commun. 2013, 438(4):753-759.
  • BMP signaling regulates the differentiation of mouse embryonic stem cells into lung epithelial cell lineages.
    Ninomiya N, Michiue T, Asashima M, Kurisaki A.
    In Vitro Cell Dev. Biol. Anim. 2013, 49(3):230-237.
  • Characterization of human adipose tissue-resident hematopoietic cell populations reveals a novel macrophage subpopulation with CD34 expression and mesenchymal multipotency.
    Eto H, Ishimine H, Kinoshita K, Watanabe-Susaki K, Kato H, Doi K, Kuno S, Kurisaki A, Yoshimura K.
    Stem Cells Dev. 2013, 22(6):985-997.


  • Novel cell surface genes expressed in the stomach primordium during gastrointestinal morphogenesis of mouse embryos.
    Noguchi TA, Ishimine H, Nakajima Y, Watanabe-Susaki K, Shigeta N, Yamakawa N, Wang PC, Asashima M, Kurisaki A.
    Gene Expr. Patterns. 2012, 12(3-4):154-163.


  • Demyelination can proceed independently of axonal degradation during Wallerian degeneration in wlds mice.
    Takada H, Yuasa S, Araki T.
    Eur J Neurosci. 2011 Aug;34(4):531-7.

Before 2010

  • TIF1beta regulates the pluripotency of embryonic stem cells in a phosphorylation-dependent manner.
    *Seki Y, *Kurisaki A, Watanabe-Susaki K, Nakajima Y, Nakanishi M, Arai Y, Shiota K, Sugino H, Asashima M.
    Proc. Natl. Acad. Sci. U S A. 2010, 107(24):10926-10931.
  • The RNA-binding protein Mex3b has a fine-tuning system for mRNA regulation in early Xenopus development.
    Takada H, Kawana T, Ito Y, Kikuno RF, Mamada H, Araki T, Koga H, Asashima M, Taira M.
    Development. 2009 Jul;136(14):2413-22.
  • Separation with zwitterionic hydrophilic interaction liquid chromatography improves protein identification by matrix-assisted laser desorption/ionization-based proteomic analysis.
    Intoh A, Kurisaki A, Fukuda H, Asashima M.
    Biomed. Chromatogr. 2009, 23(6):607-614.
  • Proteomic analysis of membrane proteins expressed specifically in pluripotent murine embryonic stem cells.
    Intoh A, Kurisaki A, Yamanaka Y, Hirano H, Fukuda H, Sugino H, Asashima M.
    Proteomics. 2009, 9(1):126-137.
  • Directed induction of anterior and posterior primitive streak by Wnt from embryonic stem cells cultured in a chemically defined serum-free medium.
    Nakanishi M, Kurisaki A, Hayashi Y, Warashina M, Ishiura S, Kusuda-Furue M, Asashima M.
    FASEB J. 2009, 23(1):114-122.
  • Physical interaction between Tbx6 and mespb is indispensable for the activation of bowline expression during Xenopus somitogenesis.
    Hitachi K, Danno H, Kondow A, Ohnuma K, Uchiyama H, Ishiura S, Kurisaki A, Asashima M.
    Biochem. Biophys. Res. Commun. 2008, 372(4):607-612.
  • Activin induces long-lasting N-methyl-D-aspartate receptor activation via scaffolding PDZ protein activin receptor interacting protein 1.
    Kurisaki A, Inoue I, Kurisaki K, Yamakawa N, Tsuchida K, Sugino H.
    Neuroscience. 2008, 151(4):1225-1235.
  • Characterization of follistatin-related gene as a negative regulatory factor for activin family members during mouse heart development.
    Takehara-Kasamatsu Y, Tsuchida K, Nakatani M, Murakami T, Kurisaki A, Hashimoto O, Ohuchi H, Kurose H, Mori K, Kagami S, Noji S, Sugino H.
    J. Med. Invest. 2007, 54(3-4):276-288.
  • Analysis of skin graft survival using green fluorescent protein transgenic mice.
    Matsuo S, Kurisaki A, Sugino H, Hashimoto I, Nakanishi H.
    J. Med. Invest. 2007, 54(3-4):267-275.
  • Dullard promotes degradation and dephosphorylation of BMP receptors and is required for neural induction.
    *Satow R, *Kurisaki A, Chan TC, Hamazaki TS, Asashima M.
    Developmental Cell. 2006, 11(6):763-774.
  • N-cadherin is a useful marker for the progenitor of cardiomyocytes differentiated from mouse ES cells in serum-free condition.
    Honda M, Kurisaki A, Ohnuma K, Okochi H, Hamazaki TS, Asashima M.
    Biochem. Biophys. Res. Commun. 2006, 351(4):877-882.
  • ALK7 is a novel marker for adipocyte differentiation.
    Kogame M, Matsuo S, Nakatani M, Kurisaki A, Nishitani H, Tsuchida K, Sugino H.
    J. Med. Invest. 2006, 53(3-4):238-245.
  • Characterization of isoforms of activin receptor-interacting protein 2 that augment activin signaling.
    Liu ZF, Tsuchida K, Matsuzaki T, Bao YL, Kurisaki A, Sugino H.
    J. Endocrinol. 2006, 189(2):409-421.
  • FKBP12 functions as an adaptor of the Smad7-Smurf1 complex on activin type I receptor.
    Yamaguchi T,Kurisaki A, Yamakawa N, Minakuchi K, Sugino H.
    J. Mol. Endocrinol. 2006, 36(3):569-579.
  • Identification of target genes for the Xenopus Hes-related protein XHR1, a prepattern factor specifying the midbrain-hindbrain boundary.
    Takada H, Hattori D, Kitayama A, Ueno N, Taira M.
    Dev Biol. 2005 Jul 1;283(1):253-67.