奈良先端科学技術大学院大学 バイオサイエンス科 膜分子複合機能学(塚崎研究室) 奈良先端科学技術大学院大学 奈良先端科学技術大学院大学 バイオサイエンス研究科 膜分子複合機能学

English

研究業績

准教授(PI) 塚崎 智也の研究業績 (Google Scholar で見る)

助教 田中 良樹の研究業績

2013年度以降(塚崎研究室発足後)に発表した論文

  1. Tanaka Y, Iwaki S. and Tsukazaki T.  Crystal Structure of a Plant Multidrug and Toxic Compound Extrusion Family Protein. Structure 25, 1455-1460 (2017).
  2. Sugano Y, Furukawa A, Nureki O, Tanaka Y, *Tsukazaki T. SecY-SecA fusion protein retains the ability to mediate protein transport.  PLOS ONE 12, e0183434 (2017).
  3. Furukawa A, Yoshikaie K, Mori T, Mori H, Morimoto VY, Sugano Y, Iwaki S, Minamino T, Sugita Y, Tanaka Y and *Tsukazaki TTunnel Formation Inferred from the I-Form Structures of the Proton-Driven Protein Secretion Motor SecDF Cell Rep. 19, 895-901 (2017).
  4. Taniguchi R, Inoue A, Sayama M, Uwamizu A, Yamashita K, Hirata K, Yoshida M, Tanaka Y, Kato HE, Nakada-Nakura Y, Otani Y, Nishizawa T, Doi T, Ohwada T, Ishitani R, Aoki J and Nureki O.  Structural insights into ligand recognition by the lysophosphatidic acid receptor LPA(6). Nature 548, 356-360 (2017) 

  5. Nishima W, Mizukami W, Tanaka Y, Ishitani R, Nureki O and Sugita Y.  Mechanismsfor Two-Step Proton Transfer Reactions in the Outward-Facing Form of MATE Transporter. Biophys J. 110, 1346-1354 (2016).
  6. Kusakizako T, Tanaka Y, Hipolito CJ, Kuroda T, Ishitani R, Suga H, *Nureki O. LCP crystallization and X-ray diffraction analysis of VcmN, a MATE transporter from Vibrio cholerae. Acta Crystallogr. F 72, 552-557 (2015)
  7. Tanaka Y, Sugano Y, Takemoto M, Mori T, Furukawa A, Kusakizako T, Kumazaki K, Kashima A, Ishitani R, Sugita Y, *Nureki O and *Tsukazaki TCrystal Structures of SecYEG in Lipidic Cubic Phase Elucidate a Precise Resting and a Peptide-Bound State Cell Rep. 13, 1561-1568 (2015) [naister]
  8. Shimokawa-Chiba N, Kumazaki K, Tsukazaki T, Nureki O, Ito K and Chiba S. Hydrophilic microenvironment required for the channel-independent insertase function of YidC protein. Proc. Natl. Acad. Sci. USA 112, 5063-5068 (2015). [naistar]
  9. Kumazaki K, Chiba S, Takemoto M, Furukawa A, Nishiyama K, Sugano Y, Mori T, Dohmae N, Hirata K, Nakada-Nakura Y, Maturana AD, Tanaka Y, Mori H, Sugita Y, Arisaka F, Ito K, Ishitani R, *Tsukazaki T and *Nureki O. Structural basis of Sec-independent membrane protein insertion by YidC. Nature 509, 516-520 (2014).  [naistar]
  10. Kumazaki K, Kishimoto T, Furukawa A. Mori H, Tanaka Y, Dohmae N, Ishitani R, *Tsukazaki T and *Nureki O. Crystal structure of Escherichia coli YidC, a membrane protein chaperone and insertase. Sci. Rep. 4, 7299 (2014). [naistar]
  11. Kumazaki K, *Tsukazaki T, Nishizawa T, Tanaka Y, Kato HE, Nakada-Nakura Y, Hirata K, Mori Y, Suga H, Dohmae N, Ishitani R and *Nureki O. Crystallization and preliminary X-ray diffraction analysis of YidC, a membrane-protein chaperone and insertase from Bacillus halodurans. Acta Crystallogr. F  70, 1056-1060 (2014).  [naistar]
  12. Mio K, Tsukazaki T, Mori H, Kawata M, Moriya T, Sasaki Y, Ishitani R, Ito K, *Nureki O and *Sato C. Conformational variation of the translocon enhancing chaperone SecDF.
    J. Struct. Funct. Genomics,15, 107-115 (2014).   [naistar]
  13. Tanaka Y, Hipolito CJ, Maturana AD, Ito K, Kuroda T, Higuchi T, Katoh T, Kato HE, Hattori M, Kumazaki K, Tsukazaki T, Ishitani R, *Suga H and *Nureki O. Structural basis for the drug extrusion mechanism by a MATE multidrug transporter. Nature 496, 247-251(2013).  [naistar]
  14. Doki S, Kato HE, Solcan N, Iwaki M, Koyama M, Hattori M, Iwase N, Tsukazaki T, Sugita Y, Kandori H, Newstead S, *Ishitani R and *Nureki O. Structural basis for dynamic mechanism of proton-coupled symport by the peptide transporter POT. Proc. Natl. Acad. Sci. USA 110, 11343-11348 (2013).  [naistar]

2012年度以前のスタッフの代表論文

  1. 【SecDFの構造と機能解析】
    Tsukazaki T, Mori H, Echizen Y, Ishitani R, Fukai S, Tanaka T, Perederina A, Vassylyev DG, Kohno T, Maturana AD, *Ito K, and *Nureki O. Structure and function of a membrane component SecDF that enhances protein export. Nature 474, 235-238 (2011)
  2. 【SecYEの構造と機能解析】
    Tsukazaki T, Mori H, Fukai S, Ishitani R, Mori T, Dohmae N, Perederina A, Sugita Y, Vassylyev DG, *Ito K and *Nureki O. Conformational transition of Sec machinery inferred from bacterial SecYE structures. Nature 455, 988-991 (2008).
  3. 【マグネシウムトランスポータの構造解析】
    Hattori M, Tanaka Y, Fukai S, Ishitani R and *Nureki O. Crystal structure of the MgtE Mg2+ transporter. Nature, 448 1072-1075 (2007).
  4. 【SecA ATPaseの構造と機能解析】
    *Vassylyev DG, Mori H, Vassylyeva MN, Tsukazaki T, Kimura Y, Tahirov TH and Ito K. Crystal structure of the translocation ATPase SecA from Thermus thermophilus reveals a parallel, head-to-head dimer. J. Mol. Biol. 364, 248-258 (2006).
  5. 【SecYEの精製と機能解析】
    Mori, H., Tsukazaki, T., Masui, R., Kuramitsu, S., Yokoyama, S., Johnson, A.E., Kimura, Y., Akiyama, Y. and Ito, K., Fluorescence resonance energy transfer analysis of protein translocase. SecYE from Thermus thermophilus HB8 forms a constitutive oligomer in membranes. J. Biol. Chem. 278, 14257-14264 (2003).