奈良先端科学技術大学院大学 バイオサイエンス科 膜分子複合機能学(塚崎研究室) 奈良先端科学技術大学院大学 奈良先端科学技術大学院大学 バイオサイエンス研究科 膜分子複合機能学

English

メンバー

助教 田中 良樹 Yoshiki Tanaka

学歴

  • 修士(理学);東京工業大学
  • 博士(理学);東京大学 平成24年

職歴

  • 平成22年
    日本学術振興会特別研究員DC2
  • 平成24年
    東京大学 大学院理学系研究科 特任研究員
  • 平成24年
    京都大学 医学研究科 特定研究員
  • 平成25年
    奈良先端科学技術大学院大学 バイオサイエンス研究科 助教

発表論文

  1. Daimon Y, Iwama-Masui C, Tanaka Y, Shiota T, Suzuki T, Miyazaki R, Sakurada H, Lithgow T, Dohmae N, Mori H, *Tsukazaki T, *Narita S and *Akiyama Y. The TPR domain of BepA is required for productive interaction with substrate proteins and the β-barrel assembly machinery complex. Mol. Microbiol. 106, 760-776 (2017).
  2. Tanaka Y, Iwaki S. and *Tsukazaki T.  Crystal Structure of a Plant Multidrug and Toxic Compound Extrusion Family Protein. Structure 25, 1455-1460 (2017).
  3. Sugano Y, Furukawa A, Nureki O, Tanaka Y, *Tsukazaki T. SecY-SecA fusion protein retains the ability to mediate protein transport.  PLOS ONE 12, e0183434 (2017)
  4. Furukawa A, Yoshikaie K, Mori T, Mori H, Morimoto VY, Sugano Y, Iwaki S, Minamino T, Sugita Y, Tanaka Y, *Tsukazaki T. Tunnel Formation Inferred from the I-Form Structures of the Proton-Driven Protein Secretion Motor SecDF Cell Rep. 19, 895-901 (2017).
  5. Taniguchi R, Inoue A, Sayama M, Uwamizu A, Yamashita K, Hirata K, Yoshida M, Tanaka Y, Kato HE, Nakada-Nakura Y, Otani Y, Nishizawa T, Doi T, Ohwada T, Ishitani R, Aoki J and Nureki O.  Structural insights into ligand recognition by the lysophosphatidic acid receptor LPA(6). Nature 548, 356-360 (2017) 
  6. Nishima W, Mizukami W, Tanaka Y, Ishitani R, Nureki O, Sugita Y.  Mechanismsfor Two-Step Proton Transfer Reactions in the Outward-Facing Form of MATE Transporter. Biophys J. 110, 1346-1354 (2016).
  7. Kusakizako T, Tanaka Y, Hipolito CJ, Kuroda T, Ishitani R, Suga H, *Nureki O. LCP crystallization and X-ray diffraction analysis of VcmN, a MATE transporter from Vibrio cholerae. Acta Crystallogr. F 72, 552-557 (2015).
  8. Tanaka Y, Sugano Y, Takemoto M, Mori T, Furukawa A, Kusakizako T, Kumazaki K, Kashima A, Ishitani R, Sugita Y, *Nureki O and *Tsukazaki T. Crystal Structures of SecYEG in Lipidic Cubic Phase Elucidate a Precise Resting and a Peptide-Bound State Cell Rep. 13, 1561-1568 (2015) [naister]
  9. Kumazaki K, Kishimoto T, Furukawa A. Mori H, Tanaka Y, Dohmae N, Ishitani R, *Tsukazaki T and *Nureki O. Crystal structure of Escherichia coli YidC, a membrane protein chaperone and insertase. Sci. Rep. 4, 7299 (2014).
  10. Kumazaki K, *Tsukazaki T, Nishizawa T, Tanaka Y, Kato HE, Nakada-Nakura Y, Hirata K, Mori Y, Suga H, Dohmae N, Ishitani R and *Nureki O. Crystallization and preliminary X-ray diffraction analysis of YidC, a membrane-protein chaperone and insertase from Bacillus haloduransActa Crystallogr. F  70, 1056-1060 (2014).
  11. Kumazaki K, Chiba S, Takemoto M, Furukawa A, Nishiyama K, Sugano Y, Mori T, Dohmae N, Hirata K, Nakada-Nakura Y, Maturana AD, Tanaka Y, Mori H, Sugita Y, Arisaka F, Ito K, Ishitani R, *Tsukazaki T and *Nureki O. Structural basis of Sec-independent membrane protein insertion by YidC. Nature 509, 516-520(2014).
  12. Tanaka Y, Hipolito CJ, Maturana AD, Ito K, Kuroda T, Higuchi T, Katoh T, Kato HE, Hattori M, Kumazaki K, Tsukazaki T, Ishitani R, Suga H and Nureki O. Structural basis for the drug extrusion mechanism by a MATE multidrug transporter. Nature 496, 247-251 (2013).
  13. Hipolito CJ, Tanaka Y, Katoh T, *Nureki O and *Suga H.  A macrocyclic peptide that serves as a cocrystallization ligand and inhibits the function of a MATE family transporter.  Molecules. 18, 10514-10530 (2013)
  14. Hattori M, Iwase N, Furuya N, Tanaka Y, Tsukazaki T, Ishitani R, Maguire ME, Ito K, Maturana AD and Nureki O. Mg2+-dependent gating of bacterial MgtE channel underlies Mg2+ homeostasis. EMBO J. 28, 3602-3612 (2009).
  15. Higuchi T, Hattori M, Tanaka Y, Ishitani R and Nureki O. Crystal structure of the cytosolic domain of the cation diffusion facilitator family protein. Proteins 76, 768-771 (2009)
  16. Tanaka Y, Hattori M, Fukai S, Ishitania R and Nureki O. Crystallization and preliminary X-ray diffraction analysis of the cytosolic domain of the Mg2+ transporter MgtE. Acta Crystallogr. F, 63(Pt 8), 678-681 (2007).
  17. Hattori M, Tanaka Y, Fukai S, Ishitani R and Nureki O. Crystallization and preliminary X-ray diffraction analysis of the full-length Mg2+ transporter MgtE. Acta Crystallogr. F, 63(Pt 8), 682-684 (2007).
  18. Hattori M, Tanaka Y, Fukai S, Ishitani R and Nureki O. Crystal structure of the MgtE Mg2+ transporter. Nature 448, 1072-1075 (2007).
  19. Hattori M, Tanaka Y, Ishitani R and Nureki O. Crystallization and preliminary X-ray diffraction analysis of the cytosolic domain of a cation diffusion facilitator family protein. Acta Crystallogr. F, 63(Pt 9), 771-773 (2007).

総説など

  1. Tanaka, Y, Ishitani R and Nureki O. Crystal structure of multidrug transporter MATE. SPring-8 Research Frontiers 2013, 24-25 (2014).
  2. 田中良樹・濡木 理, "薬剤耐性細菌の出現の原因となる多剤排出輸送体の構造および機能の解明" 新着論文レビュー, http://first.lifesciencedb.jp/archives/6953 (2013)
  3. 田中良樹, "薬剤耐性病原菌出現の原因となる多剤排出輸送体MATEの結晶構造解析" 結晶学会誌, 55 (4), 248 (2013)