セミナー情報

セミナー情報

1) Unravelling cluster root development in white lupin
2) THE ARABIDOPSIS lasso1 MUTANT LINKS ROOT GRAVITROPIC RESPONSE AND SALT STRESS

演題 1) Unravelling cluster root development in white lupin
2) THE ARABIDOPSIS lasso1 MUTANT LINKS ROOT GRAVITROPIC RESPONSE AND SALT STRESS
講演者 1) Dr Benjamin Péret
2) Dr Fanchon Divol
使用言語 English
日時 平成30年5月17日(木曜日) 15:30~17:00
場所 Large Seminar Room
内容

1) Plants show a strong level of developmental plasticity that is controlled by a complex combination of perception, integration and response. Root systems are a fantastic tool to study this plasticity since the number and position of lateral roots is deeply altered by the environment. We are trying to understand the fundamental mechanisms governing lateral root development and its control by the environment. Our research focuses on two main biological systems: the model plant Arabidopsis thaliana and white lupin (Lupinus albus). Our main project (ERC Starting grant LUPIN ROOTS) aims at understanding the formation of cluster roots in white lupin. These roots are specific lateral roots that are dedicated towards efficient phosphate acquisition and are produced as a response to its deficiency. From a developmental point of view, they consist in the induction of numerous rootlet primordia that will emerge to produce a “bottlebrush”-like structure. We believe that studying these extraordinary structures will help us understand plant organ formation as a response to their environment. Beyond its fantastic root development, white lupin is a model of interest because of its high protein seed content and because as a Legume it interacts with rhizobial bacteria to form nitrogen-fixing nodules but does not engage in mycorhizal associations.

In order to understand how cluster roots are formed, we described their development by classical histology approaches. We also set up “hairy root” transformation to use auxin markers and focus on auxin-realted genes (Gallardo et al., 2018). In parallel, we generated and started to screen an EMS mutagenized population. We have identified constitutive cluster root mutants and we are now trying to find the genes responsible for their phenotype. In 2017, we sequenced white lupin genome and generated a high-quality assembly at the chromosome level (2n=50). The genome size is 450Mb and the N50 of the final assembly is 17Mb. Also, we have performed detailed timecourse transcriptomics analysis by RNAseq that we plan to use for establishing Gene Regulatory Networks in order to find important candidates regulating cluster root formation.

Gallardo, C., Hufnagel, B., Casset, C., Alcon, C., Garcia, F., Divol, F., Marquès, L., Doumas, P., and Péret, B. (2018). Anatomical and hormonal description of rootlet primordium development along white lupin cluster root. Physiol Plant, in press

2) Plants display considerable developmental plasticity in response to changing environmental conditions. The adaptations of the root system to variations of growth conditions are an excellent model to study developmental plasticity. In an effort to identify mutants altered in root perception to salt stress, we screened an activation tagging collection (Weigel et al. 2000) and identified the lasso1 mutant that displays a strong agravitropic root growth on standard medium. Interestingly, the lasso1 mutant phenotype is partially reverted by salt and osmotic stress (sorbitol) application. The aux1 mutant, harboring a mutation in the auxin influx transporter AUX1 (Bennett et al. 1996), displays a strikingly similar root agravitropic phenotype. We demonstrated that (i) lasso1 is not allelic to aux1, (ii) the AUX1 protein localization is not altered in lasso1, and (iii) neither salt nor osmotic stress alter aux1 agravitropic root growth. We thus make the assumption that different molecular mechanisms are responsible for the phenotypic behavior of lasso1 and aux1. Auxin transport is at the core of root gravitropic response, so we analyzed the perturbation of the auxin response marker DR5::GFP in the root tips of lasso1. Our results suggest a strong accumulation of auxin in the root columella and lateral root cap and we are now focusing on the auxin efflux transporters from the PIN family. Segregation studies have demonstrated that the lasso1 phenotype is independent of the activation tagging T-DNA and therefore the sequencing of lasso1 genome is in progress to search for the origin of the mutation. This study will help us to identify new molecular mechanisms involved in the perception and integration by roots of their environment (gravity, salt, water).

問合せ先 植物発生シグナル
郷達明 (goh@bs.naist.jp)

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