Laboratories

Cell Signaling

Prof. Shiozaki
Professor
Kaz SHIOZAKI
Assistant Professor
Hisashi TATEBE
Labs HP
http://bsw3.naist.jp/shiozaki/

Outline of Research and Education

Our research aims to elucidate intracellular signaling networks that sense and transmit diverse extracellular stimuli, with particular focus on the signaling pathways involved in cancerous cell proliferation and metabolic syndromes such as diabetes. To identify and analyze novel components of the signaling pathways, the studies utilize the fission yeast Schizosaccharomyces pombe (Fig.1), which has been successfully used as a genetically amenable model system to investigate cellular regulatory mechanisms conserved from yeast to humans. Students in our laboratory are encouraged to design multifaceted approaches that logically combine research tools in molecular genetics, cell biology and biochemistry.
Originally established in 1998 at University of California-Davis, our laboratory has been training researchers that serve the international scientific community.

Major Research Topics

TOR (Target Of Rapamycin) signaling pathway

TOR kinase forms a protein complex called TORC2, which mediates insulin-induced activation of Akt kinase and cellular uptake of glucose (Fig.2). Defects in insulin signaling result in type 2 diabetes and therefore, comprehensive understanding of this pathway is crucial for the development of informed strategies to treat the disease.

Stress-responsive MAP kinase cascade

Stress-activated protein kinase (SAPK) is a member of the MAP kinase family that plays pivotal roles in cellular stress responses, including those of cancer cells exposed to cytotoxic therapies. Our goal is to discover cellular “stress sensors” that transmit signals to induce activation of SAPK.

References

  1. Hatano T. et al., Cell Cycle, 14, 848-856, 2015
  2. Fruita K. et al., J. Biomol. NMR, 61, 55-64, 2015
  3. Morigasaki S, Shiozaki K.,Commun. Integr. Biol., 6, e25020, 2013
  4. Morigasaki S. et al., Mol. Biol. Cell, 23, 1083-1092, 2013
  5. Komeda C. et al., Biomol. Chem.11, 2567-2570, 2013
  6. Tatebe H. et al., Curr. Biol., 20, 1975-1982, 2010
  7. Tatebe H. and Shiozaki K., Small GTPases, 1, 180-182, 2010
  8. Morigasaki S. and Shiozaki K., Meth. Enzymol., 471, 279-289, 2009
  9. Shiozaki K., Sci. Signal., 2, pe74, 2009
  10. Morigasaki S. et al., Mol. Cell, 30, 108-113, 2008
  11. Tatebe H. et al., Curr. Biol., 18, 322- 330, 2008
  12. Ikeda K. et al., Cell Cycle, 7, 358-364, 2008
  13. Wang L. and Shiozaki K., FEBS Lett, 580, 2409-2413, 2006
  14. Tatebe H. et al., Curr. Biol., 15, 1006-1015, 2005
  15. Wang L. et al., Mol. Cell. Biol., 25, 3945-3955, 2005
  16. Ikner A. and Shiozaki K., Mutation Res., 569, 13-27, 2005
  17. Tatebe H. and Shiozaki K., Mol. Cell. Biol., 23, 5132-5142, 2003
  18. Nguyen A. N. et al., Mol. Biol. Cell., 13, 2651-2663, 2002
  19. Santos J. L. and Shiozaki K., Science’s STKE, 98, re1, 2001
  20. Nguyen A. N. et al., Mol. Biol. Cell, 11, 1169-1181, 2000
  21. Nguyen A. N. and Shiozaki K., Genes Dev., 13, 1653-1663, 1999
  22. Shiozaki K., Shiozaki M. and Russell P., Mol. Biol. Cell, 9, 1339-1349,1998
  23. Shiozaki K., Shiozaki M. and Russell P., Mol. Biol. Cell, 8, 409-419,1997
  24. Shiozaki K, and Russell P., Meth. Enzymol., 283, 506-520,1997
  25. Shiozaki K. and Russell P., Genes. Dev., 10, 2276-2288, 1996
  26. Shiozaki K. and Russell P., Nature, 378, 739-743, 1995
  27. Shiozaki K. and Russell P., EMBO J., 14, 492-502, 1995
  28. Shiozaki K. et al., Mol. Cell. Biol., 14, 3742-3751, 1994
  29. Shiozaki K. and Yanagida M., J. Cell. Biol., 119, 1023-1036, 1992
  30. Shiozaki K. and Yanagida M., Mol. Cell. Biol., 11, 6093-6102, 1991
Fig.1 Fission yeast Schizosaccharomyces pombe.
Fig.1 Fission yeast
Schizosaccharomyces pombe.
Fig.2 TOR complex 2 (TORC2) mediates insulin signals that induce cellular uptake of glucose.
Fig.2 TOR complex 2 (TORC2)
mediates insulin signals that induce cellular uptake of glucose.
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